摘要
利用气质联用仪(GC-MS)分析了茶树精油(TTO)的成分,采用体外试验对其抗氧化和抗癌等生理功能进行了评价;用不同浓度(2 ml/L、5 ml/L、15 ml/L)的TTO对南美白对虾进行保鲜处理,评价不同处理对冷藏对虾品质的影响。结果表明:TTO的主要成分为萜品烯-4-醇、γ-萜品烯、#-萜品烯、1,8-桉叶素、萜品油烯、ρ-伞花烃、#-萜品醇;该精油具有很好的清除O.2-和.OH的能力;在1.0μl/ml浓度下即可表现出较高的抑制肝癌细胞(HepG2)增殖的能力;用2 ml/L、5 ml/L TTO浸泡处理能明显抑制对虾冷藏期间pH值、挥发性盐基氮(TVB-N)和细菌总数(TBC)的上升和感官质量的下降;但是过高的浓度(如15 ml/L),反而会对对虾保鲜产生不利影响。
In order to investigate the bioactivity of tea tree oil （ TTO）, the main components of TI＇O was measured by gas chromatography-mass spectrometry （ GC-MS）, and the antioxidant and anticancer abilities of TTO were evaluated in vitro. The quality of prawn treated with different concentrations （2 ml/L, 5 ml/L and 15 ml/L） of TTO were also assessed during cold storage at 4℃ for 5 d. The major components of TTO is Terpinen-4-ol, γ-Terpinene, α-Terpinene, 1,8-Cineole, Terpinolene, ρ-Cymene and α-Terpineol. TTO exhibited stronger capacity in clearing O2- and ℃OH in comparison with propyl gallate, and showed significant anticancer ability against the proliferation of hepatoma cell （HepG2） even at 1μl/ml concentration （2 ml/L or 5 ml/L）. TFO could inhibit the increases of pH, total volatile basic nitrogen （TVB-N） and total bacterial count （TBC） and maintain better sensory characteristics in prawn at the end of cold storage. However, higher concentration （15 ml/L） might lead to adverse effects.
出处
《江苏农业学报》
CSCD
北大核心
2013年第1期172-177,共6页
Jiangsu Journal of Agricultural Sciences
基金
国家自然科学基金项目(31271943)
浙江省自然科学基金项目(Y12C00009)
宁波市自然科学基金(2012A610143)
宁波大学学科项目(XK1119)
关键词
茶树精油
生物活性
对虾
保鲜
tea tree oil
bioactivity
prawn
preservation
