摘要
研究西红花花瓣总黄酮的提取最优条件及抗氧化活性。采用L(934)正交试验设计,考察了提取温度、时间、乙醇浓度、提取次数等因素对西红花花瓣总黄酮提取效果的影响。采用.OH自由基、.DPPH自由基体系,对西红花花瓣总黄酮的抗氧化活性进行研究。结果表明最佳提取条件为,乙醇浓度为60%,提取温度为70℃,提取时间为2 h,提取次数为3次。抗氧化结果表明,西红花花瓣总黄酮对.OH自由基、.DPPH自由基均有较好的清除作用,并与浓度呈一定依赖关系。最优条件下总黄酮得率达20.451 mg/g,西红花花瓣总黄酮对两种自由基均有不同程度的清除作用。
The extraction technology optimized by orthogonal design L9 （34）, while flavonoid of Crocus Sativus Petals adopted as indexes. Factors of temperature, extraction time, ethanol concentration, and extraction frequency were evaluated for their effects on extraction process. The antioxidant activities of flavonoid extracts were evaluated by the scavenging power of two free radicals like ~ DPPH and ~ OH. The results showed that the best extraction technology was 60 % ethanol, 2 hour of reflux, and 3 times of extraction. The content of falconoid reached 20.451 mg/g. Antioxidant results indicated that the scavenging effect on two free radicals by flavonoid extracts were obvious, and the scavenging effects depended on the concentration of the flavonoid extracts.
出处
《食品研究与开发》
CAS
北大核心
2013年第2期29-33,共5页
Food Research and Development
关键词
西红花
总黄酮
正交试验
抗氧化活性
Crocus Sativus
flavonoid
orthogonal design
antioxidant activity
