摘要
目的探讨左、右归丸增加去势大鼠阴道固有层血管数量的机制。方法将雌性育龄期大鼠随机分为正常组、假手术组、去势模型组、去势倍美力组、去势左右归丸组。正常组、假手术组、去势模型组:每日生理盐水灌胃;去势倍美力组:以62.5ug/100g倍美力灌胃;去势左右归丸组:按成人用量的15倍灌胃。连续给药12周。采用HE染色法对阴道组织进行病理切片制作,通过Mias-2000图像分析系统测量阴道粘膜厚度、上皮层数、皱襞数和阴道固有层血管数;采用荧光定量PCR技术检测阴道组织促血管生成素-1(Ang-1)、促血管生成素-2(Ang-2)、碱性成纤维生长因子(bF-GF)mRNA表达水平。结果与假手术组、正常组比较,去势模型组大鼠阴道粘膜厚度、阴道皱襞数、阴道固有层血管数明显降低(P<0.05);与去势模型组比较,去势倍美力组大鼠阴道粘膜厚度、阴道皱襞数、阴道固有层血管数明显增加(P<0.05),去势左、右归丸组大鼠阴道皱襞数、阴道固有层血管数均明显升高(P<0.05)。假手术组、正常组、去势倍美力组、去势左、右归丸组大鼠阴道组织Ang-1mRNA的表达量分别为模型组的2.73、2.50、5.50、6.78、4.46倍。去势倍美力组、去势左、右归丸组大鼠阴道组织Ang-2mRNA的表达量分别为模型组2.44、3.06、2.32倍。假手术组、正常组、去势倍美力组、去势左、右归丸组Ang-1/Ang-2mRNA比值为2.18、2.66、2.25、2.22、1.92倍。假手术组、正常组、去势倍美力组、去势左、右归丸组大鼠阴道组织bFGFmRNA的表达量分别为模型组1.97、1.74、2.02、2.73、1.92倍。结论左右归丸通过调节去势雌性大鼠阴道Ang-1/Ang-2mRNA比值、促进bFGFmRNA表达而促进去势雌性大鼠阴道固有层血管数量。
Objective This study was to investigate the mechanism of increasing vessel numbers of vaginal lamina propria in ovariectomized rats by Zuoguiwan （ZGW） and Youguiwan （YGW）. Methods Female sexual matured rats were randomly divided into 6 groups： normal group, sham-operated group, model group, Premarin group, ZGW and YGW group. Normal group, sham-operated group, model group were fed by saline. Rats in Premarin group were fed by Premarin （62.5ug/100g） ; Rats from ZGW and YGW group were fed by the 1-5 formula respectively in dosage of 15 folds of adults. All rats were fed for 12 weeks. The thickness of vaginal mucosa, covering epithelium, vaginal folds and vessel numbers of lamina propria were measured by Mias-2000 image analysis system. The expression of vaginal Ang-1, Ang-2, bFGF and RNA were measure by fluorescent quantitation PCR. Results Compared with sham-operated and normal groups, the thickness of vaginal mucosa, vaginal folds and vessel numbers of lamina propria in model group were in- creased significantly （P〈0.05） ; Compared with model group, the thickness of vaginal mucosa, vaginal folds and vessel numbers of lamina propria in Premarin group were increased significantly （P〈（0. 05）, the thickness of vaginal folds and vessel numbers of lamina propria in ZGW and YGW group were increased significantly （P〈0. 05）. Expression of vaginal Ang-lmRNA in sham-operated, normal, Premarin, ZGW and YGW groups were 2.73, 2.50, 5.50, 6.78, 4.46 folds of model group respectively. Expression of vaginal Ang-2mRNA in Premarin, ZGW and YGW groups were 2.44, 3.06, 2.32 folds of model group respectively. The Ang-1/Ang-2mRNA ratio in Sham group, normal group, Premarin, EGW and YGW groups were 2.18, 2.66, 2.25, 2.22, 1.92. Expression of vaginal bFGFmRNA in Sham group, normal group, Premarin, ZGW and YGW groups were 1.97, 1.74, 2.02, 2. 73, 1.92 folds of model group respectively. ConclusionZGW and YGW might improve vessel numbers of vaginal lamina propria, by regulating vaginal Ang-1/Ang-2mRNA ratio and promoting the expression of bFGFmRNA in ovariectomized rats.
出处
《西部医学》
2013年第3期337-340,共4页
Medical Journal of West China
基金
国家自然科学基金(30873279)
国家重点基础研究发展(973)计划(2010CB530403)
