摘要
目的 探讨小剂量前列腺素F２α和E２（PGF２α、PGE２)对正常兔眼压的作用及与环磷酸腺苷(cAMP)的房水动力学介导作用的相关性。方法 采用随机盲法对照研究，30只兔60只眼随机分成三组，每组分别点用PGF２α10μg或PGE２ １０μg或等量生理盐水。每组选10眼，用药后不同时间测量眼压。另外10眼滴药后2h抽取房水检测cAMP含量变化。结果 PGF２α治疗组用药后0..5h、PGE２组用药后1h眼压开始下降，持续至用药后5h，其中2h时眼压下降幅度最大，平均分别为0..50kPa和0..45kPa。用药2h后PGE２组房水中cAMP含量由用药前(17..50±3..80)pmol/ml增高至(37..21±4.34)pmol/ml，而PGF２α组房水cAMP含量变化不明显。结论 小剂量PGF２α及PGE２可以降低正常兔眼内压。PGE２降眼压机理与房水中第二信使cAMP的动力学介导作用相关，而PGF２α可能另有一套不同的第二信使系统。
Objective\ To investigate the effect of topical administ ration of prostaglandin F２α ,E2 (PGF２α,PGE２) in small dosage on the normal rabbits' eyes an d the hydrodynamic mediation of cyclic AMP(cAMP) in aqueous humor. Methods\ In randomized fashion,60 eyes of 30 rabbits were divided into 3 groups.Eyes in al l groups were administrated topically with one drop of 10μg PGF２α,or 10 μg PGE２or equal 0.9% saline solution.Intraocular pressure (IOP) were mea s ured on 10 eyes of every group at different times.The cAMP production in aqueous humors were determinated in another 10 eyes in 2h after administration. Results\ IOP began to decrease in 0.5h after administration in PGF２α group and i n 1h in PGE２ group.The effect lasted for 5h and reached a maxinum in 2h, average 0.50kPa and 0.45kPa respectively.The cAMP production in aqueous humor wa s increased from (17.5±3.80)mmol/L of preadministration to (37.21±4.34) mmol/L i n PGE２group,but the change of cAMP was not obvious in PGF２α group . Conclusion\ Small dosage of PGF２α,PGE２ may decrease n ormal rabbits' IOP.The mechanism of PGE２ is related to the hydrodynamic m ediation of the second message cAMP in aqueous humor and PGF２α probably has a different second messenger system.\=
出处
《西安医科大学学报》
CSCD
2000年第5期465-467,共3页
Journal of Xi'an Medical University(Chinese)
基金
院科研基金资助项目!(No.9618)
