摘要
目的 :研究绿色荧光蛋白 (GFP)在前列腺癌 PC- 3M细胞中表达及对其细胞周期的影响。方法 :制备含绿色荧光蛋白基因的重组 DNA,用阳离子脂质体 Lipofectin Regant转染培养的 PC-3M细胞 ,在倒置荧光显微镜下观察 GFP的表达情况 ,同时经流式细胞仪测定阳性克隆的细胞周期。结果 :转染后有 1 0 %～ 1 5%细胞表达绿色荧光蛋白 ,且阳性克隆细胞与亲本细胞的细胞周期无明显改变。结论
? Objective:To reseach the expression of green fluorescent protein (GFP) gene in prostate carcinoma PC 3M cell line and the efffect of cell cycle.Methods:GFP gene recombination DNA was constructed and PC 3M cells were transfected with lipofectin regant.The expression of GFP was observed under invert fluorescence microscope.Meanwhile,the cell cycle of positive cell colon was assayed by flow cytometer.Results:After transfection,about 10%~15% cells expressed green fluorescent protein and there was no significant change compared the cell cycle of positive cell colon with parents cells.Conclusion:GFP supplys a new way in reseaching metabolic phenomenon of live cells.
出处
《白求恩医科大学学报》
CSCD
2000年第6期592-594,共3页
Journal of Norman Bethune University of Medical Science
关键词
绿色荧光蛋白
报告基因
脂质体
前列腺癌
green fluorescent protein
reporter gene
lipofectin regant
prostate
