摘要
目的研究细胞周期对ＰＣ３Ｍ细胞表面Ｆａｓ／ＡＰＯ１蛋白表达的影响。方法采用直接免疫荧光流式细胞术对前列腺癌细胞系ＰＣ３Ｍ未同步化的细胞，及经改良的笑气阻断及ＴｄＲ双阻断方法同步化的不同细胞周期细胞表面Ｆａｓ／ＡＰＯ１表达情况进行检测。结果未同步化的ＰＣ３Ｍ细胞Ｆａｓ／ＡＰＯ１表达阳性率为３４２０％；ＰＣ３Ｍ细胞系不同细胞周期的细胞表面Ｆａｓ／ＡＰＯ１表达明显不同，以Ｓ期最高达８５０５％，Ｇ１及Ｍ期较低，分别为３３２％和４９５％。结论细胞周期对细胞表面Ｆａｓ／ＡＰＯ１的表达情况有明显影响。深入研究细胞周期对肿瘤细胞Ｆａｓ／ＡＰＯ１表达情况的影响，对于采用Ｆａｓ／ＡＰＯ１系统途径治疗恶性肿瘤有重要意义。
Objective To determine the influence of cell cycle on the expression of Fas/APO 1 in PC 3M cell line. Methods With direct immunofluorescence flow cytometry,expression of Fas/APO 1 in unsynchronized and synchronized cells of PC 3M cell line derived from non androgen dependent prostatic carcinoma was detected. Results Unsynchronized cells of PC 3M cell line expressed Fas/APO 1 in 34.20 %;expression levels of Fas/APO 1 in the cells of PC 3M in different cell cycles being significantly different,mainly expressed in S phase (positive rate reached 85.05%),and limitedly expressed in G 1 and M phases (positive rate was 3.32% and 4.95% respectively). Conclusions Cell cycle would influence the expression level of Fas/APO 1 significantly,and might have some clinical relevance.
出处
《中华泌尿外科杂志》
CAS
CSCD
北大核心
1999年第4期237-239,共3页
Chinese Journal of Urology
