摘要
目的观察小檗碱对血管性痴呆大鼠炎性细胞因子TNF-α、IL-1β、IL-8表达水平的影响,探讨血管性痴呆炎症损伤机制及小檗碱对血管性痴呆大鼠脑保护作用机制。方法采用2-血管阻断方法制作血管性痴呆模型。随机分为正常组、假手术组、模型组、小檗碱治疗组(低、中、高剂量治疗组)。Morris水迷宫实验检测大鼠的空间学习以及记忆能力;ELISA法检测大鼠海马区TNF-α、IL-1β、IL-8表达水平。结果与正常组比较,模型组、治疗组海马区TNF-α、IL-1β、IL-8表达水平明显升高(P<0.05)。与模型组比较,治疗组的行为学症状较模型组明显改善,治疗组海马区TNF-α、IL-1β、IL-8表达水平明显下降(P<0.05),并存在剂量依赖现象。结论炎症反应在血管性痴呆的发生中起到重要的作用,小檗碱抑制炎性的表达存在剂量依赖现象。小檗碱能改善血管性痴呆大鼠的行为学症状,小檗碱可能是通过降低炎性因子(如TNF-α、IL-1β、IL-8等)来抑制炎症反应的途径而发挥脑保护作用,使血管性痴呆症状得到改善。
Objective To observe the influence of berberine on expression of TNF-α,IL-1β,IL-8 in rats with vascular dementia, so as to study the inflammation damage mechanism of vascular dementia and the neu- roprotective mechanism of berberine for vascular dementia. Methods An animal model of vascular dementia was established by two-Vessel occlusion. Animals were randomly divided into normal group, sham operation group, model group, berberine group（low, middle, high dose group）. The behaviors of animals were tested with Morris water maze. Enzyme linked immunosorbent assay（ELISA） was used to observe the expression of TNF-α,IL-1β, IL-8 in hippocamp. Results Compared to normal group： The learning and memory of model group, berberine group were lower compared with normal group（P〈0. 05） ; there was no difference between sham operation group and normal group（P〈0. 05）. The expression of TNF-α,IL-1β,IL-8 in model group, ber- berine group（low, middle, high dose group） were higher（P〈0. 05）. Compared to model group： The learning and memory of berberine group（low, middle, high dose group） were improved. The expression of TNF-α,IL- 1β,IL-8 in berberine group（low, middle, high dose group） were lower（P〈0. 05）. Conclusions Inflammatory reaction plays an important role in the development of vascular dementia, berberine reduces inflanmaatory me- diator express dose-dependently. Berberine improves the learning and memory in VI） rats, its effects may be correlated with the decrease in hippocampal inflammatory mediator（TNF-α,IL-1β,IL-8）.
出处
《卒中与神经疾病》
2013年第1期16-19,共4页
Stroke and Nervous Diseases
