摘要
目的 探讨鼠伤寒沙门氏菌 porin(STM- porin)诱导 BAL B/ c小鼠细胞免疫的能力。方法 迟发型变态反应(DTH)、IL - 2分别采用足垫肿胀、MTT法 ;利用尼龙毛柱纯化免疫的 BAL B/ c小鼠的 T淋巴细胞通过尾静脉转移到非免疫小鼠 ,再用 5 0 0 L D5 0 的 STM攻击。结果 用 STM- porin免疫的 BAL B/ c小鼠可以产生较高水平的 DTH (3.6± 0 .2 ,P<0 .0 1)和IL - 2 (2 6 .2± 4.9,P<0 .0 1) ;同时 T淋巴细胞被动免疫保护 (42 .9% )也明显高于 L PS组 (0 % ) ,P<0 .0 1。结论 这些结果说明 STM- porin可能诱导 BAL B/
Aim\ To investigate cell mediated-immunity induced by porin of Salmonella typhimurium(STM).Methods Level of delayed-type hypersensitivity(DTH),IL-2 of immunized BALB/c mice were determined by footpad swelling ,MTT and transfered protective rate of T cells purified by neloy wool were studied with 500LD 50 of the bacteria(intraperitoneally).Results A marked level of DTH(3.6±0.2,P<0.01),IL-2(26.2±4.9,P<0.01) and the immuinized T cells transfer protective level (42.9%,P<0.01) was induced by porin from STM.Conclusion These results indicated that porin of STM might induced stronger CMI in BALB/c mice.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第6期45-47,共3页
Chinese Journal of Zoonoses
关键词
伤寒沙门氏菌porin
BALB/C
细胞免疫
小鼠
Salmonella typhimurium
Porin
Delayed-type hypersensitivity IL-2
T cell transfer immuity
