摘要
为开发高效实用的EST-SSRs标记,从120605条辣椒无冗余EST序列中共搜索到10179条至少含有1个SSR的EST序列,占EST总数的8.44%。高频重复类型为一核苷酸、二核苷酸和三核苷酸,占EST-SSRs总数的91.63%。最常见的基元是A/T,其次是TC/GA和CT/AG。设计合成了20对EST-SSRs引物并对25份辣椒材料进行了PCR扩增,结果表明:17对引物在供试辣椒材料中能扩增出明显的条带,其中12对引物扩增出42条多态性条带,平均多态性条带为3.5条,引物的多态性信息含量介于0.21～0.95之间。利用EST-SSRs标记对供试品种的聚类分析结果与形态学、生物学分类结果基本一致,表明本研究中开发的EST-SSRs标记可用于辣椒种质资源的遗传多样性分析。
To develop efficient and practical EST-SSRs markers, 10 179 EST sequences each of which contained at least one SSR were obtained from 120 605 non-redundant EST in pepper, accounting for 8.44% in all EST. Mononucleotide, dinucleotide and trinuleotide were the main repeat types with a percentage of 91.63% in all EST-SSRs. A/T was the most frequent motif with a frequency of 31.17%, followed by TC/GA and CT/AG, accounting for 10.0% and 9.97% respectively. Twenty pairs of EST-SSRs primers were designed and synthesized and were used to amplify 25 pepper varieties. The results showed that 17 pairs of primers could amplify clear bands, and 12 out of 17 pairs could amplify 42 polymorphic bands with an average of 3.5 bands per pair of primer. The polymorphic information content of primers screened were between 0.21 and 0.95. The clustering results of 25 pepper varieties based on EST-SSRs markers were in agreement with morphological and biological classification, it is indicated that EST-SSRs markers developed in the study could be used for germplasm resources genetic diversity analysis in pepper.
出处
《园艺学报》
CAS
CSCD
北大核心
2013年第2期265-274,共10页
Acta Horticulturae Sinica
基金
公益性行业(农业)科研专项(201203004)
甘肃省农业科学院农业科技创新专项(2011GAAS06-15)
甘肃省青年科技基金项目(1208RJYA039)
