摘要
目的:在无菌培养条件下探讨植物激素对滇重楼种子休眠破除及植株形态发生的影响。方法:以滇重楼成熟种子为试验材料,在无菌培养条件下采用正交试验设计研究赤霉素(GA3)、玉米素核苷(ZR)、吲哚乙酸(IAA)及活性炭(AC)对滇重楼种子休眠破除的影响,并对无菌萌发过程中植株形态发生进行观察。结果:滇重楼种子无菌培养过程中种子萌动的最佳配比是GA3-ZR-IAA-AC(1.0 mg.L-1∶1.0 mg.L-1∶0.1 mg.L-1∶0.5 g.L-1),种子萌动率达80.00%,4种因子对滇重楼种子萌动率作用大小为IAA>GA3>ZR>AC;滇重楼种子无菌培养过程中种子萌发的最佳配比是GA3-ZR-IAA-AC(1.0 mg.L-1∶1.0 mg.L-1∶0.1 mg.L-1∶0.5 g.L-1),种子萌发率达66.66%,4种因子对滇种楼种子发芽率作用大小为AC>IAA>ZR>GA3。结论:培养基添加植物激素GA,ZR,IAA,AC对滇重楼种子休眠破除、种子萌动及萌发有重要影响。
Objective：To explore the effect of phytohormone on the breaking seed dormancy and plant morphogenesis of Paris polyphylla var.yunnanensis under aseptic culture condition. Method： The mature seeds of P.polyphylla var.yunnanensis were used for the experimental materials. The effects of gibberellic acid （GA3）, trans-zeatin-riboside （ZR）, 3-indolyl-acetic acid （IAA）, and active carbon （AC） on the breaking seed dormancy of P.polyphylla var.yunnanensis were investigated under aseptic culture condition by orthogonal experimental design, at the same time, during the aseptic germination process the plant morphogenesis was observed. Result： The best ratio for the seed protrusion of P.polyphylla var.yunnanensis was GA3-ZR-IAA-AC 1.0 mg·L-1∶1.0 mg·L-1∶0.1 mg·L-1∶0.5 g·L-1, and seed protrusion rate is up to 80.00%. The role sequence of four factors on the seed protrusion rate of P.polyphylla var.yunnanensis has been found to decrease in the order： IAA〉GA3〉ZR〉AC. The best ratio for the seed germination of P.polyphylla var.yunnanensis is： GA3-ZR-IAA-AC 1.0 mg·L-1∶1.0 mg·L-1∶0.1 mg·L-1∶0.5 g·L-1, and seed germination rate is up to 66.66%. The role sequence of four factors on the seed germination rate of P.polyphylla var.yunnanensis was found to decrease in the order： AC〉IAA〉ZR〉GA3. Conclusion： Adding the phytohormone GA, ZR, IAA, and AC into the culture medium has very important influence on the breaking seed dormancy, seed protrusion and germination of P.polyphylla var.yunnanensis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第5期161-164,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
云南省应用基础研究项目(2010ZC136)
大理州科技项目2011年州院共建洱海污染矣江实验基地建设项目
大理学院2011年中青年学术带头人培养项目
关键词
滇重楼
种子休眠
植物激素
形态发生
Paris polyphylla var.yunnanensis
seed dormancy
phytohormone
morphogenesis
