摘要
目的研究T一2毒素对低硒（Se）大鼠关节软骨抗氧化酶及基因表达的影响。方法新生断乳雄性健康sD大鼠，平均体重60-80g，随机分成两大组即常规组和低se组喂养30d，低Se动物模型建造成功后再分成常规组、低se组、常规＋低T一2组、常规＋高T．2组、低se＋低T一2组、低se＋高T一2组，再T～2毒索灌胃饲养30d后，观察低se、T一2毒素及二者共同作用对各组大鼠关节软骨丙二醛（MDA）含量、总抗氧化能力（T—AOC）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH—Px）活性及SOD、CAT、GSH—PxmRNA表达的影响。结果与常规组相比，各组大鼠关节软骨中MDA含量升高，T—AOC、SOD、CAT和GSH—Px活性下降，均有统计学意义（P〈0．05）；低Se＋低T一2组、低se＋高T一2组与低se组相比，MDA含量升高，T—AoC、SOD、CAT和GSH—Px活性下降（P〈0．05）；与常规组相比，各组大鼠关节软骨SOD、CAT、GSH—PxmRNA表达降低（P〈0．05）。结论低se或单纯T一2毒素均可减弱大鼠关节软骨抗氧化酶活性，低se与T．2毒素对机体抗氧化功能有协同作用。
Objective To study the effect of T-2 toxin on articular cartilage antioxidant enzymes and gene expression in selenium （Se） dificient rats. Method Weanling male SD rats （60-80） g were randomly divided into normal group and Se-deficient group and fed for 30 d. After the animal model of Se-deficiency was developed, the rats were randomly divided into 6 groups： normal group （ I ）, Se-deficient group （II）, normal＋low T-2 group （III）, normal＋high T-2 group （IV）, Se-deficient＋low T-2 group （V）, Se-deficient＋high T-2 group （VI）. The group I ,III, V were fed normal feed, and the other groups were fed low-Se feed. All groups were given T-2 toxin at 100 or 200 ng/（g bw＇d） except the group I and II. At the 30th day of the experiment, the content of malondialdehyde （MDA）, the total antioxidant capacity （T-AOC） and activities of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase（GSH-Px） and SOD, CAT, GSH-Px mRNA expression were observed. Results The MDA content in the other groups was significantly higher than group I （P〈0.05）. Besides, the T-AOC and activities of SOD, CAT, GSH-Px in the other groups were significantly lower than group I （P〈0.05）. Compared with group II, the content of MDA in group V and VI was increased and the T-AOC, activities of SOD, CAT, GSH-Px were decreased （P〈0.05） . Compared with group I, the mRNA expression of SOD, CAT, GSH-Px in the other groups were significantly lower （P〈0.05） . Conclusion Se-deficiency or T-2 toxin could weaken the activities of articular cartilage antioxidant enzymes. Se-deficiency and T-2 toxin had synergistic effect on the antioxidant function.
出处
《营养学报》
CAS
CSCD
北大核心
2013年第1期64-67,共4页
Acta Nutrimenta Sinica
基金
国家自然科学基金重点项目(No.30630058)
陕西省自然科学基金(No.SJ08~ZT12)
第四军医大学"2012年度精英人才系列资助计划"之"优秀文职人员培养基金"(4138C4IDK9)
关键词
T-2毒素
硒
关节软骨
抗氧化酶
基因表达
T-2 toxin
selenium（Se）
arthrodial cartilage
antioxidant enzymes
gene expression
