摘要
目的:在营养剂量范围内比较低硒大鼠对亚硒酸钠和硒蛋白的生物利用效果以及剂量-反应关系。方法:雄性SD大鼠72只,按体重随机分为9组,自由进食克山病区低硒饲料。6周后开始对低硒大鼠补硒,对照组每天灌胃纯水;亚硒酸纳、硒蛋白各4组,每天灌胃相应的硒强化剂(各分四个硒剂量2、4、8、16μg/kg bw)。连续灌胃4周后处死采样,通过血肝肾中的硒含量以及肝肾组织中谷胱甘肽过氧化物酶(GSH-Px)活性和硫氧还蛋白还原酶(TrxR)活性来比较生物利用效果。结果:与对照组相比,血肝肾中的硒含量以及肝肾组织中GSH-Px活性和TrxR活性均随补硒剂量的增加而增加(P<0.05),呈量效关系。在同一补硒水平上,亚硒酸钠组大鼠肝肾组织中的硒含量高于硒蛋白组(P<0.05)。在4g/kg bw剂量时,硒蛋白组大鼠肾GSH-Px活性高于亚硒酸钠(P<0.05)。在16g/kg bw剂量时,硒蛋白组大鼠肾TrxR活性高于亚硒酸钠(P<0.05)。结论:硒蛋白增加大鼠肝肾组织中的硒含量低于亚硒酸钠,但对含硒酶的影响却优于亚硒酸钠,因此硒蛋白能更好的被大鼠吸收利用。
Objective：To compare the bioavailability between sodium selenite and selenoprotein in low selenium rats in the rang of nutrition level and study the dose-response relationship.Methods：72 Male SD rats were divided randomly into 9 groups according to weight,control group,Na2SeO3 4 groups and selenoprotein groups.All rats were fed in the selenium deficient diet in Keshan disease areas for 6 weeks.Control group rats were given purified water.Na2SeO3groups and selenoprotein groups were given selenium enriched substance including different dosage（2、4、8、16μg/kg bw）by intragastric administration for 4 weeks.Selenium content,glutathione peroxidase（GSH-Px）activity and thioredoxin reductase（TrxR）activity were tested to compare bioavailability.Results：Compared with control group,both selenium contents in blood,liver and kidney and the activity of GSH-Px and TrxR in liver and kidney were enhanced significantly with the increasing of selenium（P〈0.05）.The selenium contents of liver and kidney in Na2SeO3groups were higher than selenoprotein groups in the same selenium level.However,the activity of GSH-Px and TrxR in selenoprotein groups were higher than Na2SeO3groups.Conclusion：Compared with Na2SeO3,selenoprotein can be effectively absorbed and utilized in low selenium rat.
出处
《中国食品添加剂》
CAS
北大核心
2010年第6期95-98,共4页
China Food Additives
关键词
低硒
硒蛋白
生物利用
谷胱甘肽过氧化物酶
low selenium
selenoprotein
bioavailability
glutathione peroxidase
