摘要
目的 为研究槟榔碱 (Arecoline)灭螺增效的作用机制。方法 用加入和未加入增效剂的灭螺药物浸泡钉螺 2 4h后 ,观察不同浓度的Are单用或与杀螺剂合用对钉螺中枢神经节Mg2 + -ATPase、ChE、SDH、LDH的变化。结果 6 2 5mg/LAre浸泡钉螺 2 4h后 ,其中枢神经节Mg2 + -ATPase被明显破坏 ,ChE轻度破坏 ;1 5 6mg/LAre与 1 2 5mg/LNaPCP、 0 2 5mg/LNic、 15 6 2 5mg/LSG合用时 ,对钉螺中枢神经节Mg2 + -ATPase的破坏作用大于后三种药物单用时的作用 ;各实验组SDH、LDH无明显改变 ,与正常对照组无显著性差异 (P >0 0 5 )。结论 槟榔碱的增效作用机制在于增强其它杀螺药的药效作用 ,药物的杀螺位点在于阻断氧化磷酸化偶联生成ATP ,进而影响能量代谢 ,可能最终因ATP生成和利用障碍而使钉螺丧失机械运动及生物合成等生命功能而死亡。
Aim To study the mechanisms of strengthening molluscicidal effect of Arecoline and seek for the mixed molluscicide which is more effective,low poison,low pollution and less cost Methods Observed the changes of Mg 2+ -ATPase、CHE、SDH and LDH in the centric ganglion of Oncomelania hupensis ,after being immersed in several concentration of molluscicide of chemicals or of plants combine used with adding Are and no adding Are for 24 hours Results After being immersed in 6 25mg/L.Are for 24 hours,Mg 2+ -ATPase in the centric ganglion of Oncomelania hupensis was destroyed significantly;at same time,CHE in the centric ganglion was destroyed lightly The effect of combining use of 1 56mg/L.Are with 1 25mg/L Napcp、0 25mg/L Nic and 15 65mg/L SG acting on Mg 2+ -ATPase in the centric ganglion of the snail was better than the effect of the single-use of 1 25mg/LNapcp、0 25fmg/L Nic and 15 625mg/L SG It isnt much different from the compared groups in the activity of SDH and LDH in all chemicals groups Conclusion The mechanism of Arecoling in strengthening molluscacidal effect is that the Arecoling strengthen the effect of other molluscicide,it acted on stopping the process of ATP forming in the centric ganglion of the snails and put snails to death,because of the vital movement losing and the vital anabolism stopping
出处
《中国人兽共患病杂志》
CSCD
北大核心
2000年第3期34-37,F002,共5页
Chinese Journal of Zoonoses
基金
国家自然科学基金!资助项目课题 (批准号 :39670 661 )
关键词
钉螺
血吸虫病
灭螺药增效剂
酶组织化学
Oncomelania hupensis
schistosomaiasis
molluscicidal
Mg 2+ -ATPase
ChE
SDH
LDH
