摘要
目的探讨乙二胺四乙酸盐二钾（EDTA-K2）等抗凝剂对假性血小板减少症（PTCP）的影响及解决方法。方法采用Sysmex-2100全自动血细胞分析仪检测PTCP患者的EDTA-K2抗凝血、枸橼酸钠抗凝血、肝素锂抗凝血的血小板计数值，并用手工计数检测血小板。结果EDTA—K2抗凝血的血小板计数值明显低于枸橼酸钠抗凝血及肝素锂抗凝血，枸橼酸钠抗凝血及肝素锂抗凝血的血小板计数值与末梢血手工计数法血小板结果相接近。EDTA-K2抗凝血涂片经瑞一姬染色显示有大量血小板聚集，聚集的血小板大小不一，数量不等。而枸橼酸钠抗凝血及肝素锂抗凝血涂片则无血小板聚集现象。结论EDTA—K2引起的血小板聚集可以导致血小板计数的假性减少。对于首次检测血小板减少的标本都应涂片瑞．姬染色镜检以排除或确认有无血小板聚集现象。对抗凝剂依赖的血小板减少症患者应采用末梢血手工计数法、新鲜血（不加抗凝剂）仪器旁即刻检测法等方法进行血小板检测。
Objective To study the influence and solution of pseudothrom bocytopenia （PTCP） caused by anticoagulant EDTA-K2. Methods Sysmex-2100 hematology analyzer was used to analyze PTCP-positive patients＇ blood samples, count the platelet in samples anticoagulant with EDTA-K2 , sodium citrate and heparin, and used the manual counting methods. Results Platelets counts in anticoagulant bood by EDTA-K2 was significantly lower than anticoagulant bood by sodium citrate and heparin. Lots of aggregated platelets were appeared in the EDTA-K2 anticoag- ulant samples stained by Wright-Giemsa,while there was no aggregated platelets observed in sodium citrate anticoagu- lant samples and heparin anticoagulant samples. Conclusion Platelet aggregation in patients with PTCP causes pate- let counts false reducation. The patients with PTCP is determined by measuring the whole blood （no anticoagulant）by automatic hematology analyzer immediately or manual couting method.
出处
《中国基层医药》
CAS
2013年第3期396-398,共3页
Chinese Journal of Primary Medicine and Pharmacy
关键词
抗凝药
血小板计数
现实检验
Anticoagulants
Platelet count
Reality testing
