摘要
采用黑暗摇瓶发酵和蓝光照射静置培养的两步培养法,进行蛹虫草(Cordyceps militaris L.)液体发酵产类胡萝卜素的蓝光诱导。结果表明蛹虫草在2d的黑暗培养和5d的蓝光照射静置培养后,其类胡萝卜素的含量可达到最高值558.4μg/gFW。而以黑暗摇瓶培养2d后,进行不同时间的蓝光照射静置培养。结果表明,蓝光照射最初2d,蛹虫草类胡萝卜素含量变化不明显,随后快速增加,并在第5天达到最大值558.4μg/gFW,随后类胡萝卜素的含量并无明显变化。通过研究解决了蛹虫草液体发酵产类胡萝卜素的培养过程中蓝光的给光问题。
Utilizing the dark shake flask culture and the static culture of blue light irradiation in the twostep fermentation, the blue light induced the carotenoid production of Cordyceps militaris L mycelia. The results showed that after 2 days shake flask in the darkness and 5 days static culture in the blue light, Cordyceps militaris L carotenoid content reached a maximum value 558.4μg/gFW. But adopting the dark shake flask culture of 2 days and different times static culture in blue light, the results show that for the first 2 days of blue light irradiation, the increase of Cordyceps militaris carotenoids content was not obvious, then a rapid increase was obtained and a maximum 558.4μg/gFW was reached in the fifth day, subsequently the content of carotenoids did not significantly change. The problem about imposed blue light is solved in the carotenoid production of Cordyceps militaris L fermentation.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2013年第2期83-87,共5页
China Biotechnology
基金
国家自然科学基金(31060004)
江西省自然科学基金(2010GZN0115)
江西省教育厅科技项目(GJJ11387)资助项目
关键词
蛹虫草
蓝光诱导
发酵
类胡萝卜素
Cordyceps militaris L. Blue light induction Fermentation Carotenoid
