摘要
提出了离子色谱法测定减肥食品中左旋肉碱含量的方法。样品用超纯水于30℃超声提取30 min,经离心分离,取上清液通过OnGuard II RP柱净化。流出液以IonPac CS17柱为离子交换柱,5 mmol·L^(-1)甲基磺酸溶液为淋洗液,采用非抑制电导检测器进行检测。左旋肉碱的质量浓度在10～1 000 mg·L^(-1)范围内与其峰面积呈线性关系,检出限(3S/N)为1 mg·g^(-1)。以两种减肥产品为基体,进行加标回收试验,所得回收率在95.4%～101%之间,测定值的相对标准偏差(n=5)在0.9%～6.6%之间。
A method of IC for determination of L-carnitine in slimming diet was proposed. The sample was extracted with ultrapure water for 30 min at 30℃, after centrifugation, the supernatant was purified by passing through OnGuard II RP column. IonPac CS17 column was used for separation with 5 mmol · L^- 1 methylsulfonic acid solution as eluant. Non-inhibitory conductivity detection was adopted in the determination. Linear relationship between values of peak area and mass concentration of L-carnitine was obtained in the range of 10--1 000 mg · L^-1 ,with detection limit （3S/N） of 1 mg ·g^- 1. Using 2 samples of slimming diet as matrixes, test for recovery was made by standard addition method, giving values of recovery and RSD＇s （n = 5） in ranges of 95.4%- 101% and 0. 9 % -- 6.6 % respectively.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2013年第1期6-10,共5页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
