摘要
[目的]得到能使大豆油彻底水解的复合脂肪酶配方。[方法]采用不同来源的脂肪酶对大豆油进行水解,以酸值的变化来衡量水解程度,研究了单一脂肪酶和复合脂肪酶对大豆油的水解条件。[结果]单一脂肪酶水解时质量分数2%时水解最好,水解酸值达到126 mgKOH/g油脂;使用复合脂肪酶时水解效率明显提高,达到150 mgKOH/g油脂左右。[结论]复合脂肪酶是一种既经济又很高效的脂肪酶,它可使大豆油的水解率大大提高,并且克服了脂肪酶制备成本高、易失活、转化效率不太高等缺点,可以应用到工业上,降低成本价,获得更大的利益。
[Objective] To obtain compound lipase for hydrolysis of soybean oil.[Method] Lipase from different sources was used as catalyst to hydrolysis soybean.The change of acid value was observed for measuring the degree of hydrolysis during the experiment course.The single and complex lipase hydrolysis conditions of lipase were studied.[Result] The best hydrolysis condition was the mass fraction of 2% by single lipase.The acid value could up to 126 mgKOH/g.Using compound lipase to hydrolyze,the efficiency obviously increased,reaching around 150 mgKOH/g.[Conclusion] As an economy and high-efficiency lipase,the compound lipase could be applied in industry,which not only can significantly improve soybean oil hydrolysis rate,but also can overcome disadvantages of high cost for lipase preparation,easy to lost activity,low transformation efficiency.
出处
《安徽农业科学》
CAS
2013年第2期819-820,852,共3页
Journal of Anhui Agricultural Sciences
基金
云南省工业与信息化委员会项目(2011292)
云南省应用基础研究基金项目(2010-CD050)
云南省能源局项目(20102127)
云南省教育厅科学研究基金项目(2011Z028)
关键词
大豆油
复合脂肪酶
水解率
Soybean oil
Compound lipase
Hydrolysis rate
