摘要
目的从大蒜中分离纯化具有抗真菌活性的蛋白。方法提取大蒜粗提液,通过DEAE-SephadexTM A-50阴离子交换层析、Affi-gel blue gel亲和层析和ResourceTMS阳离子交换层析纯化目的蛋白。通过Tricine-SDS-PAGE、抗真菌活性检测鉴定该蛋白对不同真菌的抑制活性。结果纯化得到的蛋白分子质量为13ku,该蛋白对落花生褐斑病菌有明显的生长抑制活性,而对绿色木霉、黑曲霉、白地霉和产黄青霉没有生长抑制活性。纯化得到另外一种蛋白的分子质量约为4.6ku,该蛋白对绿色木霉有明显的生长抑制活性,而对落花生褐斑病菌、黑曲霉、白地霉和产黄青霉没有生长抑制活性。结论大蒜中存在抗真菌活性蛋白。
Objective To isolate and purify the protein with antifungal activity from Allium sativum.Methods Crude extracts were obtained from Allium sativum and antimicrobial proteins were purified by ion exchange chromatography on DEAE-SephadexTM A-50,affinity chromatography on Affi-gel blue gel,and ion exchange chromatography on ResourceTM S.The antifungal activity of the purified proteins was identified by Tricine-SDS-PAGE and antifungal activity test.Results The molecular mass of the purified protein was 13 ku.The protein inhibited the growth of Mycosphaerella arachidicola,but had no effects on Trichoderma viride,Aspergillus niger,Geotrichum candidum and Penicillium chrysogenum.In addition,another purified protein had a molecular mass of 4.6 ku.The protein inhibited the growth of Trichoderma viride,but had no effects on Mycosphaerella arachidicola,Aspergillus niger,Geotrichum candidum and Penicillium chrysogenum.Conclusion Antimicrobial proteins isolated from Allium sativum can prohibit the growth of some fungi.
出处
《南昌大学学报（医学版）》
CAS
2012年第12期1-5,9,共6页
Journal of Nanchang University：Medical Sciences
基金
国家自然科学基金(81271950)
深圳市重点实验室组建项目(SW201110010)
关键词
大蒜
离子交换层析
亲和层析
活性抑制
Allium sativum
ion-exchange column chromatography
affinity chromatography
activity prohibition
