摘要
目的研究前列腺癌(PC)及良性前列腺增生症(BPH)组织中高分子量角蛋白抗体(34βE12)、α-甲酰基辅酶A消旋酶(P504s)、P63蛋白表达水平及其临床意义。方法采用EnVision两步免疫组织化学方法,检测69例未经任何抗癌治疗的PC和39例BPH石蜡标本中34βE12、P504s和P63蛋白的表达,分析联合检测34βE12、P504s及P63蛋白表达对PC的诊断与鉴别诊断的价值。结果 34βE12、P504s在PC的阳性表达率分别为2.8%、95.7%,P63蛋白呈阴性表达;而34βE12和P63蛋白在BPH组中的阳性表达率均为100%,P504s均为阴性表达。结论 P504s是PC敏感而特异性的标记物,联合检测P504s、34βE12和P63蛋白可提高PC的诊断准确率,且对PC的鉴别诊断有重要价值。
Objective To study the expressions of high molecular weight eytokeratin antibody（3413E12） , α-methylacyl-CoA racemase（P504s） and P63 proteins in the tissues of prostate cancer （PC） and benign prostate hyperplasia （BPH） and their clinical significance. Methods Collecting paraffin-section samples from the tissues of 69 cases of PC without any treatment and 39 cases of BPH ,the expressions of 3413E12 ,P504s and P63 proteins were detected by two-steps immunohistochemical method. The value of their combining detection in diagnosis and differential diagnosis on PC was discussed. Results The positive expression rates of 34βE12 and P504s proteins in PC were 2.8% and 95.7% respectively, while the expression of P63 was negative. The positive expression rates of 34βE12 and P63 proteins in BPH were all 100% ,while the expression of P504s was negative. Conclusions P504s is a sensitive and specific marker of PC. Combining detection of P504s,3413E12 and P63 proteins expressions could improve the diagnostic accuracy rate and have important value for differential diagnosis of PC.
出处
《中国临床研究》
CAS
2013年第2期105-107,共3页
Chinese Journal of Clinical Research
基金
河南省教育厅自然科学研究项目(2011C310005)
