摘要
目的研究白藜芦醇对脂多糖(LPS)造成的急性肺损伤的保护作用及其分子机制。方法用LPS气管内滴注构建小鼠急性肺损伤的模型。HE染色法观察肺病理形态学变化,ELISA法检测小鼠肺组织匀浆中肿瘤坏死因子(TNF-α)、白介素-6(IL-6)的含量,用Real-time PCR和ELISA分别检测硫氧还蛋白mRNA和蛋白水平表达。结果病理形态学显示白藜芦醇可明显减轻LPS诱导的肺损伤,并显著减少TNF-α,IL-6的含量。细胞水平白藜芦醇在50～100μg/ml范围内可以明显提高LPS刺激后的细胞活力。Real-time PCR和ELISA检测结果表明:白藜芦醇能明显提高动物肺组织和细胞中硫氧还蛋白的mRNA和蛋白水平。结论白藜芦醇可以减轻LPS诱导的肺损伤,其机制可能与提高Trx1有关。
Objective To investigate the mechanism and effects of resveratrol protected acute lung injury（ ALI） induced by lipopolysaccharide （LPS）. Methods Model of ALI in mice was built by LPS intratracheal instillation. Lung tissue was stains with Hematoxylin-eosin to observe the pathological. The Inflammatory cytokines TNF-α, IL-6 was assayed with ELISA in homogenates of lung tissue. Real-time PCR and ELISA were used to detect the expressions of thioredoxinl （Trxl） both in mRNA and protein level. Results In vivo, resveratrol decreased inflammatory cytokines TNF-α, IL-6 in homogenates of lung tissue, at the same time suppressed lung edema, pulmonary alveolar damage. In vitro, resveratrol （50-100 μg/ml, final concentration） could improve cells viability induced by LPS. Furthermore, resveratrol up-regulated expressions of Trxl both protein and mRNA level in vivo and in vitro. Conclusion Resveratrol can protect against ALI induced by LPS, which underlying mechanism may be relationship with up-regulated Trxl expression.
出处
《中华肺部疾病杂志（电子版）》
CAS
2013年第1期13-16,共4页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
2012年国家自然科学基金面上项目(81270124)
