摘要
目的比较苏州市两家医院女性宫颈上皮细胞中人乳头瘤病毒(human papillomavirus,HPV)感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对4393例宫颈上皮细胞标本进行23种HPV基因分型检测。结果 4393例女性宫颈上皮细胞标本,HPV总阳性率为31.64%(1390/4393),苏州大学附属第一医院和苏州市立东区医院HPV总阳性率分别为33.44%(611/1827)、30.36%(779/2566),一重感染阳性率分别为22.44%(410/1827)、20.73%(532/2566),多重感染阳性率分别为11.00%(201/1827)、9.63%(247/2566),两院HPV总阳性率及一重、多重感染阳性率比较差异无统计学意义(P>0.05)。结论基因扩增结合基因芯片检测技术可应用于宫颈细胞标本HPV分型检测,对女性宫颈HPV感染的分子流行病学研究具有重要意义。
Objective To compare the clinical distribution of human papillomavirus（HPV） genotypes in two hospitals of Suzbou city. Methods Polymerase chain reaction and gene chip technology were utilized for the detection of 23 kinds of HPV genotypes in epithelial cell specimens from 4 393 cases of women. And related materials of all subjects were analyzed. Results The total HPV positive rate was 31.64%（1 390/4 393）in all specimens. The total positive rates of HPV were 33.44% （611/1 827）and 30.36%0 （779/2 566）in First Affiliated Hospital of Suzhou University and East District Hospital of Suzhou City respectively. The positive rates of single genotype infection were 22.44%（410/1 827）and 20.73% （532/2 566）. The positive rates of multiple genotypes in-fection were 11.00% （201/1 827）and 9.63% （247/2 566）. All of the positive rates mentioned above were not significantly different between two hospitals（P〉0.05）. Conclusion Gene chip technology could detect HPV genotypes in cervical cell samples, and might be important for the study of HPV genotypes distribution among women.
出处
《国际检验医学杂志》
CAS
2013年第4期404-406,共3页
International Journal of Laboratory Medicine
基金
南京市卫生局中医专项支助项目(2009-92)
