摘要
目的人乳头瘤病毒(human papillomavirus,HPV)感染是宫颈上皮内瘤变及宫颈癌的主要致病因子。该文旨在探讨宫颈腺癌中23种人乳头瘤病毒感染的基因型分布情况及其临床意义。方法从盐城及南京地区47例宫颈腺癌及100例正常宫颈组织标本中提取23种HPV DNA,采用基因扩增结合基因芯片技术对其宫颈腺癌及正常组织进行23种HPV基因型别的检测,并对其受检者进行相关资料分析。结果 47例宫颈腺癌标本中HPV阳性者44例,感染率为93.6%(44/47);其中HPV16和(或)HPV18感染40例,感染率为85.1%(40/47);单一型别HPV感染者13例,感染率27.7%(13/47),其中HPV16型为7例,占14.9%(7/47),是最主要的感染类型;混合型感染31例,占66%(31/47),其中HPV16型+18型6例,占混合型感染的19.4%(6/31),是混合型感染最主要的类型。结论 HPV感染是宫颈腺癌发生的主要致病因素,HPV16和HPV18感染是宫颈腺癌中最常见的类型;基因扩增结合基因芯片检测技术是一种较为快速、可靠的适用于临床进行HPV分型检测诊断方法。
Objective To research the intribution of 23 genotypes of human papillomavirus（HPV）which was well known as the major cause of cervical cancer in human and its clinical significance in cervical adenocarcinoma. Methods HPV DNA was extracted from cervical tissue samples of 47 cervical adenocarcinoma patients,normal cervical tissue samples of 100 healthy people were col-lected at the same time. 23 types of HPV genotypes were detected by PCR and gene chips technique. Results The total HPV posi-tive rate was 93.6%（44/47）. The rate of HPV type 16 and/or HPV type 18 infection was 85.1%（40/47）. The rate of single type HPV infection was 27.7%（13/47） ,in which HPV type 16 was predominant,with the infection rate of 14.9%（7/47）. The rate of mixed-infection was 66 % （31/47）. There were 6 cases of mixed infection of HPV type 16 and HPV type 18, with the infection rate of 19.4% （6/31） ,which was the predominant mixed infection types. Conclusion HPV infection appears to be a major risk factor for cervical adenocarcinoma. The predominant genotypes were HPV type 16 and HPV type 18 in cervical adenocarcinoma. PCR combined with gene chip technique can detect multiple HPV genotypes in cervical tissue samples with high sensitivity and specifici-ty,which is useful in the diagnosis of HPV genotype.
出处
《国际检验医学杂志》
CAS
2013年第4期393-394,397,共3页
International Journal of Laboratory Medicine
关键词
宫颈腺癌
人乳头瘤病毒
基因分型
基因芯片
感染率
cervical adenocarcinoma
human papillomavirus
genotype
gene chip
infection rate
