摘要
[Objective] To investigate the primary and secondary metabolism during the seed germination of Scutellaria baicalensis Georgi. [Method] The activities of SOD and POD were determined with lactoflavine-NBT method and guaiacol-colorimetry as- say, respectively. The activities of CAT, APX,PAL and C4H were determined with ultraviolet spectrophotometry. The contents of secondary metabolites were detected by High-performance Liquid Chromatography （HPLC）. [Result] The results indicate that the soluble sugar content decreased during the first 5 days and then increased when the cotyledons formed. The contents of PAL, C4H and CHS continuously in- creased at different stages during the seed germination process. The secondary metabolites also showed the consistent variation trend. In addition, the contents of secondary metabolites had significant positive correlation with the key enzyme activi- ty. [Conclusion] The formation of secondary metabolites is significantly positively cor- related with the key enzyme during the seed germination process. Therefore, the key enzyme activity can be enhanced by adopting appropriate measures to improve the secondary metabolites, thereby obtaining high-quality medicinal materials.
[目的]了解黄芩Scutellaria baicalensis Georgi种子萌发过程中的初生与次生代谢规律。[方法]超氧化物岐化酶(SOD)活性测定采用核黄素-NBT法,过氧化物酶(POD)活性测定采用愈创木酚比色法,过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、苯丙氨酸解氨酶和肉桂酸-4-羟化酶活性测定采用紫外分光光度法。次生代谢物质含量测定采用高效液相色谱法。[结果]在黄芩种子萌发的前5天,可溶性糖的含量不断降低,当子叶形成时有所增高。苯丙氨酸解氨酶、肉桂酸-4-羟化酶和查耳酮合成酶均随着萌发的进行而增加,其次生代谢物质也呈现出相同的变化趋势。并且次生代谢物质与关键酶的活性呈现正相关。[结论]在种子萌发的过程中次生代谢物质的形成与关键酶呈现显著性的相关,所以我们要想获得高质量的药材可以通过采取措施提高关键酶的活性。
基金
Supported by Agricultural Improved Variety Project Program of Shandong Province(2005LZ08, 2008LZ013)~~
