摘要
在生理pH=7.4环境下,用摩尔比法测定γ-环糊精(γ-CD)与茜素黄R(AYR)的包合比(摩尔比)nγ-CD∶nAYR=1∶1,确定包合常数Kοf,288.15K=1.69×105L/mol。以吖啶橙(AO)作为分子探针,用紫外光谱法、荧光光谱法、化学热力学法和黏度法等研究包合物γ-CD-AYR与鲱鱼精DNA(hsDNA)的作用,得到γ-CD-AYR包合物与hsDNA作用的结合比nDNA∶nγ-CD-AYR=1∶2,结合常数为K288.15K=3.10×104L/mol,K310.15K=4.02×104 L/mol。热力学函数ΔrHmο=8.78×103J/mol,ΔrGmο=-2.59×104 J/mol,ΔrSmο=116.45 J/(mol.K),说明γ-CD-AYR包合物与DNA作用为熵驱动。确定γ-CD-AYR与hsDNA的作用方式为静电和部分嵌插的作用方式。
Under the condition of physiological environment at pH 7.4, the binding ratio n.t.CD： nAyR =1：1 and binding constant K ,28s 15K 1.69 105 L/mol were confirmed by mole ratio method and linear method. AO was used as molecule probe, and the interaction between -CD-AYR and herring-sperm DNA was studied by UV spectra, fluorescence spectra, thermodynamic method and viscosity method, etc. A series of thermodynamic data such as ArHm = 8.78 X 103J/mol, ArGm = -2. 59 x 104 J/tool, ArSme = 116.45 J/ （mol K） were obtained, and ArSm was the primary driven power of this interac- tion, the binding ratio was nDNA： n_CD_AyR = 1：2, the binding constants were K288.15K = 3.10 104 L/mol, g310.15K = 4. 02 104 L/mol. The interaction mode between ＂-CD-AYR and herring-sperm DNA was an electrostatic interaction and part embedded intercalation.
出处
《中山大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第1期89-93,共5页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家自然科学基金资助项目(30973634)
关键词
茜素黄R
Γ-环糊精
包合物
鲱鱼精DNA
作用方式
alizarin yellow R
y-cyclodextrin
inclusion complex
herring-sperm DNA
interactionmode
