摘要
根据NCBI中提供的番茄红素单碱基突变基因hp1和dg的序列,设计特异性引物。利用同一PCR反应体系同时扩增筛选番茄红素基因hp1和dg,以及野生型基因HP1和DG。结果表明:扩增的特异性片段与单基因引物扩增片段完全吻合。SNP分子标记为单显性标记,突变基因纯合体只在突变材料PCR体系中产生298和870bp的特异性片段,野生基因纯合体只在野生材料PCR体系中产生298和870bp的特异性片段,杂合体在2个反应体系中均有特异性片段。利用该体系在F2代中筛选出同时含hp1和dg基因的纯合植株,大大节省了工作量,提高了准确度。
The specific primers were designed according to the sequence of lycopene single base mutation gene hp1 and dg.The lycopene gene hp1 and dg,as well as the wild-type gene HP1 and DG were amplified using a multi-PCR reaction system.The results showed that specific fragments fit perfectly with the single gene amplification fragments.SNP was a single dominant marker,homozygote of mutant gene in mutation material PCR reaction system producex only a specific fragment of 298 and 870 bp,and homozygote of the wild gene coulx produce the specific amplified fragment in wild material PCR reaction system,but heterozygotes could amplify both of the two specific fragments.The hp1 and the dg gene homozygous plants could be screened in the F2 generation using this system.The detection technology could greatly save the workload,and improve accuracy.
出处
《北方园艺》
CAS
北大核心
2013年第4期99-102,共4页
Northern Horticulture
基金
国家科技部"863"计划资助项目(2007AA10Z-178)
