摘要
采用超声波辅助提取鱼鳞胶原蛋白,对提取工艺以及胶原蛋白的体外抗氧化性进行了研究。结果表明:超声波辅助提取鱼鳞胶原蛋白的最佳工艺条件为超声波作用时间230 min,酶用量350 mg/g,提取液浓度0.3 mol/L,料液比1:45(g/mL),在该条件下,其提取率为28.53%;采用化学比色法测定胶原蛋白在体外的总抗氧化能力、抑制活性超氧阴离子自由基(O_2^-·)的能力、清除羟自由基(·OH)的能力。结果表明:一定浓度范围内的胶原蛋白均有总抗氧化能力,能清除O_2^-·和·OH,且呈现一定剂量关系。
With the scales as the raw materials, using ultrasonic to extraction its collagen, for its extraction technology and collagen in vitro oxidation resistance was studied. The results showed that： the best technological conditions of ultrasonic extraction auxiliary scales collagen was ultrasonic action time 230 min, the dosage of enzyme 300 mg/g, the extract concentration 0.3 mol/L, and liquid materials 1 ： 45 g/mL. In this condition, the extraction rate was 28.53%. In addition, using chemical colorimetric method to determination collagen＇s extracorporeal total antioxidant capacity; the capacity to inhibit the activity of superoxide radical （02-＇）; the ability to remove hydroxyl radicals （.OH）. The results showed that the certain concentration range of collagen protein were always oxidation resistance, and it could remove O,-＂ and .OH a dose relationshio.
出处
《食品工业》
北大核心
2013年第2期57-60,共4页
The Food Industry
基金
西昌学院研究生项目资助(XY09-2A02)
关键词
鱼鳞
胶原蛋白
超声波
抗氧化性
scales
collagen
ultrasonic
oxidation resistance
