摘要
通过比较不同提取方法及设计正交试验确定了蝉拟青霉中虫草素的最佳提取工艺:采取管式离心机离心发酵醪,收集菌丝体50℃热空气烘干,按菌粉:石油醚=1:1的量用石油醚脱脂,用布氏漏斗抽滤收集菌丝体烘干,最后用75%乙醇、45℃超声波浸提虫草素,提取4次,每次提取40min,料液比为1:20。
According to the extraction effects of different solvents,the physical and chemical characters tests of cordycepin,the optimal extraction condition was determined as follows：（1） centrifuging the fermenting matter with centrifugal machine and collecting the mycelium to dry by hot air at 50 ℃.（2） degreasing the fungus powder with petroleum ether according to fungus powder：the benzine=1：1＇s quantity.（3） using 75% ethyl alcohol,45 ℃ supersonic extracting cordycepin,and the extraction run four times with the raw material-water ratio at 1/20 and it lasted 40 minutes each time.
出处
《食品科技》
CAS
北大核心
2013年第2期218-221,共4页
Food Science and Technology
基金
安徽省农科院青年基金项目(12B0617)
国家蚕桑产业技术体系项目(CARS-22-SYZ09)
安徽省农科院创新团队项目(11C0610)
关键词
蝉拟青霉
虫草素
提取工艺
Paecilomyces cicadae
cordycepin
extraction condition
