摘要
目的:对皱皮木瓜籽的抗氧化能力进行评价,并观察其对人肝癌细胞生长抑制作用,寻找皱皮木瓜籽的潜在功能。方法:用80%乙醇进行超声提取木瓜籽,提取物浓缩后以正己烷、乙酸乙酯分别萃取。采用福林-肖卡法和铝盐显色法测定各组分的总酚和总黄酮含量。并利用ORAC法、电化学法和细胞氧化应激法评价各组分抗氧化能力,MTT法分析各组分对肝癌细胞的生长抑制作用。结果:在各种抗氧化评价方法下,乙酸乙酯组分均具有最好的抗氧化能力且该组分总酚和总黄酮含量最高,分别为(1012.24±79.16)mg/100g、(267.65±20.07)mg/g,推测可能多酚黄酮是皱皮木瓜籽中主要的抗氧化物质。而在抑制HepG2细胞生长的活性方面,正己烷组分抑制作用最为明显,IC50值为344.8μg/mL。结论:皱皮木瓜籽提取物具有较强的抗氧化能力且对肝癌细胞的生长具有一定生长抑制作用,是开发抗氧化以及抗癌产品的潜在资源。
Objective：To evaluate the potential activitives of Chinese quince seed by studing the antioxidant activity and growth inhibition for HepG2 cells.Method：Chinese quince seed wereultrasound extracted by 80% ethanol.The antioxidant activities of then-butanol,ethyl acetate and water factions from the ethanol extractof seeds were assessed by ORAC,electrochemical and cellular oxidative stressmethod.The content of total phenolics and total flavonoids were detected by Folin-Ciocalteu＇s reagent method and NaNO 2-AlCl 3 colorimetric method.MTT was used to observe cell growth inhibition effect of three extracts.Results：Ethyl acetate extract had a better antioxidant capacity as well as the highest content of total phenolics and total flavonoids,thecontent of which were（1012.24±79.16） mg/100 g and（267.65±20.07） mg/g respectively.We presume that phenolics and flavonoids are the major antioxidants in Chinese quince seed.Inthe aspect of growth inhibition of HepG2 cells,the IC 50 of n-butanol extract was 344.8 μg/mL.Conclusion：Chinese quince seed extract not only has strong antioxidant activitives but also inhibited the growth of HepG2 cells.The results provided strong envidence for the development of antioxidant and anticancerproducts by Chinese quince seed.
出处
《食品科技》
CAS
北大核心
2013年第2期207-210,217,共5页
Food Science and Technology
关键词
皱皮木瓜籽
总酚
总黄酮
抗氧化
HepG2细胞生长抑制
Chinese quince seed
total phenolics
flavonoids
antioxidant
growth inhibition of HepG2 cell
