摘要
目的了解福建省Ⅰ型艾滋病病毒(HIV-1)的耐药基因变异情况。方法选取2005-2012年福建省抗病毒治疗1年以上、病毒载量高于1 000拷贝/mL的HIV感染者/艾滋病病人标本,进行基因型耐药性检测。同时收集前期研究中的耐药性检测数据进行对比分析。结果共检测73例病毒学治疗失败病人标本,其中60例(82.19%)成功扩增序列,结合125份未接受抗病毒治疗者的样本,共获得185份样本进行耐药分析。未治疗组和治疗组对蛋白酶抑制剂(PI)的耐药发生率均较低,分别为1.60%(2/125)和8.33%(5/60);未治疗组对反转录酶抑制剂(RTI)的耐药发生率为4.80%(6/125);治疗组对RTI耐药发生率较高,为61.67%(37/60),尤其是对NVP、EFV、3TC和FTC。最常见的耐药突变有M184V、D67N、K70R、K219Q、T215F、G190A、Y181C和K103N。结论福建省HIV-1感染者的总体原发性耐药突变率较低,病毒学治疗失败病人对PI耐药发生率仍处于低水平,但对RTI耐药发生率较高。
Objective To investigate the presence of drug resistance mutations of human immunodeficiency virus type 1 （HIV-1） strains in Fujian Province. Methods HIV drug resistance mutations were tested among the HIV/ AIDS patients with viral load above 1 000 copies/mL who were receiving antiretroviral therapy （ART） for more than one year in Fujian Province from 2005 to 2012. Meanwhile, the resistance mutation data were collected for a com- parative analysis and a total of 125 samples from ART naive patients were also collected. Results Seventy three specimens of patients with therapeutic failure were identified, of which 60 cases （82.2%） were successful in se- quence amplification with polymerase chain reaction （PCR）. In combination with 125 ART naive cases, a total of 185 samples were analyzed, and the ratios of protease inhibitor （PI） resistance between the ART naive and the trea- ted groups were 1.60% （2/125） and 8.33% （5/60）, respectively. The ratio of reserve transcriptase inhibitor （RTI） resistance in the ART naive group was 4.80% （6/125）, while that in the ART treated group was 61.67% （37/60）, higher than in the ART naYve group, especially to NVP, EFV, 3TC and FTC. Commonly observed re- sistance mutations were M184V, D67N, K70R, K219Q, T215F, G190A, Y181C and K103N. Conclusion The o- verall prevalence of HIV-1 primary resistance was at a low level among the ART naive patients in Fujian Province. The ratio of PI resistance was low, but RTI was higher among patients with therapeutic failure.
出处
《中国艾滋病性病》
CAS
2013年第1期6-9,共4页
Chinese Journal of Aids & STD
基金
福建省自然科学基金(2009J01104)~~
