摘要
目的应用全细胞膜片钳研究P2受体介导对大鼠的三叉神经节(TG)神经元上电压门控性钙离子通道(VGCC)的作用。方法急性分离大鼠TG神经元细胞,通过全细胞膜片钳记录电压门控性钙离子通道电流,分别灌流腺嘌呤核苷三磷酸(ATP)及P2X、P2Y受体的激动剂,观察其对VGCC电流的作用。结果 ATP对大鼠TG神经元细胞的VGCC电流有可逆的抑制作用(P<0.05),P2X受体激动剂αβ-meATP能够显著抑制TG神经元的VGCC电流(P<0.05),而P2Y受体激动剂2-MeSADP对VGCC电流无明显作用。结论 P2受体介导对大鼠的TG神经元上VGCC电流有抑制作用。
Objective To investigate the effect of P2 receptor on voltage-gated calcium channels in trigeminal ganglion （TG） neurons. Methods Acutely isolated TG neurons were used. Voltage-gated calcium channel （VGCC） currents were recorded by patch clamp technique. Adenosine triphosphate （ATP） , P2X and P2Y receptor agonists were perfused respectively to observe the role of VGCC currents. Results ATP could reversibly inhibit the VGCC currents of rat TG neurons （P 〈 0. 05 ）. oL[3-methylene ATP （αβ-meATP） and a P2X receptor agonist could significantly inhibit the VGCC currents of rat TG neurons （P 〈 0.05）. However, 2-MethylthioADP （2-MeSADP）, a P2Y receptor agonist, had no effect on VGCC currents of rat TG neurons. Conclusion P2X receptors mediate inhibitory effect on the VGCC currents in rat.
出处
《安徽医科大学学报》
CAS
北大核心
2013年第3期207-210,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81271162)
安徽省自然科学基金(编号:11040606M204)
上海市博士后科研基金(编号:10R21415000)
