摘要
目的:建立简便快捷的高效液相色谱法测定感冒灵颗粒中对乙酰氨基酚含量。方法:采用Agilent1100型高效液相色谱仪,Agilent Eclipse XDB C18柱(250 mm×4.6 mm,5μm),流动相为甲醇-水(1∶3),流速1.0 ml.min-1,检测波长为240 nm。结果:对乙酰氨基酚进样量在4.004～36.036μg.ml-1范围内与峰面积线性关系良好(r=0.999 9),平均回收率为99.93%,RSD为0.29%(n=6)。结论:高效液相色谱法测定感冒灵颗粒中对乙酰氨基酚含量操作简便,灵敏度高,重现性好,结果精确可靠。
Objective： To establish a simple and fast high performance liquid chromatography（HPLC） determination method to detect the acetaminophen content in Ganmaoling particles.Methods： Agilent 1100 liquid chromatograph and Agilent Eclipse XDB C18 column（250mm×4.6mm,5μm） were used.Mobile phase was methanol-water（1∶3）,flow rate was 1.0ml·min^-1,detection wavelength was 240 nm.Results： The peak area of the acetaminophen injection volume in the range of 4.004-36.036μg·ml-1 was with a good linear relationship（r=0.9999）,the average recovery rate was 99.93%,RSD was 0.29%（n=6）.Conclusion： HPLC is simple,high sensitivity,good reproducibility,accurate to detect the acetaminophen content Ganmaoling particles.It can have reliable results.
出处
《现代医学》
2013年第2期124-126,共3页
Modern Medical Journal
关键词
感冒灵颗粒
对乙酰氨基酚
高效液相色谱法
Ganmaoling particles
acetaminophen
high performance liquid chromatography
