摘要
目的:研究汗衣台体外抗乙型肝炎病毒(hepatitis B virus,HBV)的疗效.方法:不同浓度汗衣台与体外培养的2.2.15细胞共同孵育,以XTT方法检测其对2.2.15细胞的不良反应,以此决定汗衣台的安全浓度范围;3d更换一次含药培养液,9d后收集上清液;用ELISA法测定上清液中HBsAg和HBeAg含量,荧光定量PCR法检测上清液中HBV DNA的分泌.结果:各浓度汗衣台对HBsAg、HBeAg均有明显抑制作用,抑制率呈剂量依赖性:500-800μg/mL汗衣台对HBsAg的抑制明显高于同浓度的拉米夫定(P=0.002-0.000)及干扰素(P=0.006-0.003),100-800μg/mL浓度汗衣台对HBeAg的抑制明显高于同浓度拉米夫定(P=0.002-0.000)及干扰素(P=0.003-0.002),差异均有显著性.各浓度汗衣台对HBV DNA抑制亦呈剂量依赖性,500-800μg/mL浓度汗衣台短期内抑制HBV DNA分泌的疗效优于同浓度干扰素(P=0.018-0.031),但不如拉米夫定.结论:壮药汗衣台具有一定的体外抗HBV作用.
AIM： To assess whether the Chuang herb Hanyitai has anti-HBV activity in vitro. METHODS： XTT assay was used to detect the cytotoxicity of different concentrations of Hanyitai in HepG2215 cells to determine the safe concentration range. Cell culture medium was changed once every 3 days, and culture supernatants were collected after 9 days of culture. The contents of HBsAg and HBeAg in supernatants were measured using ELISA. Meanwhile, the secretion of HBV-DNA was detected using fluo- rescent quantitative PCR.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第2期171-176,共6页
World Chinese Journal of Digestology
基金
广西自然科学基金资助项目
No.2010GXNSFA013198
广西中医药管理局中医药科技专项课题基金资助项目
No.GZKZ09-11
广西教育厅科研基金资助项目
No.200810LX002~~
