摘要
按回捕日期取样测定长江口中华绒螯蟹亲蟹标志放流前后血清与肝胰腺的生理指标,初步研究了放流亲蟹对长江口环境的生理适应过程。结果表明,在放流后6d,亲蟹肝胰腺超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性以及血清甘油三酯(TG)和血蓝蛋白含量均降低,SOD、CAT和TG在放流后9d达到较低水平,其中SOD活性和TG含量较放流前显著降低(P<0.05);肝胰腺酸性磷酸酶(ACP)以及血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、碱性磷酸酶(ALP)、总蛋白质(TP)、白蛋白(ALB)、总胆固醇(TC)和肌酐(CREA)水平在放流后总体呈现先升高后降低的趋势;在放流后79d,肝胰腺SOD、CAT、ACP与血清ALP、ALT、AST活性均恢复至放流前水平(P>0.05),而血清TP、血蓝蛋白、ALB、TC、TG、CREA含量较放流前均显著降低(P<0.05)。综合各项指标表明,中华绒螯蟹亲蟹在放流后6d内出现免疫力下降、代谢增强等反应,放流22d后亲蟹各项机能逐步恢复,并在70d后接近或达到放流前水平。建议今后放流前对亲蟹进行环境适应性驯化,以便提高亲蟹增殖放流效果。
This study investigated the physiology adaption of the mature Eriocheir sinensis to the environment of the Yangtze estuary after stocking by tagging method.Thirty thousand mature crabs were released in the Yangtze estuary on December 23,2010,and five thousand of which were tagged both by the label tag and the ringlike tag.The fishing tools of the recapture were 1.5-meter-high fishing nets,whose mesh size was 10 cm2.The daily fishing time depended on the ebb tide time of the Yangtze estuary,and the fishing mesh was pulled up at the end of the ebb in the daytime.Simultaneously,the data was recorded,which included the number of the crabs and the temperature,the salinity pH and the dissolved oxygen of the water and so on.We tested and recorded some physiological and biochemical indices of the hepatopancreas and the serum of the crabs before and after stocking,which were recaptured in different days.If the crabs’ number of the same day and place more than 20,we selected 20 crabs randomly as the samples to test the indices.However,if the number is less than 20,all the crabs were selected.To reduce the impact to the indices,which was made by the complex environments of Yangtze estuary,the samples of the crabs that were captured in the similar area was selected out and divided into 6 groups according the captured day.And set the samples of the carbs before stocking as the control group.There were 7 groups in the experiment,which were 0d,6d,9d,13d,22d,70d,and 79d group.Then compared and analyzed the data with SPSS statistic software.The result showed that the indices had some changes after the crabs being released.After having been released for 6 days,the activities of superoxide dismutase（SOD） and the catalase（CAT） in the hepatopancreas and the concentration of the triglyceride（TG） and the hemocyanin in the serum of the mature crabs were reduced on the 6th day after.And then three days later,the SOD,CAT activities and TG concentration of the mature crabs all declined to a very low level.And at this time,the SOD activity and TG concentration of the mature crabs were significantly different with those before stocking（P0.05）.After stocking,the activity of acid phosphatase（ACP） in the hepatopancreas and the activities（or concentrations） of alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,alkaline phosphatase（ALP）,hemocyanin,total protein（TP）,albumin（ALB）,total cholesterol（TC）,creatinine（CREA） in the serum of the mature crabs all increased first and then declined broadly.On the 79th day after stocking,the SOD,CAT,ACP activities in the hepatopancreas and the ALP,ALT,AST activities in the serum of the mature crabs all resumed to a similar level with those before being released（P0.05）.However,the TP,hemocyanin,ALB,TC,TG,CREA activities in the serum were significantly lower than those before stocking（P0.05）.Through the research,we found that the mature Eriocheir sinensis had series of reaction after stocking,such as the immunity declined and the metabolism enhanced.The metabolism and the function of the organs and tissues of the mature crabs were resumed step by step after having been released for 22 days.And they all reached the similar level with those of the crabs before stocking on the 70th day.The result showed that it may take the mature crabs 22 days to adapt the environment of the Yangtze estuary.In order to strengthen the environmental adaptability and the stock enhancement effect of the Eriocheir sinensis,good nutrition diets and related environmental acclimation should be provided.
出处
《水生生物学报》
CAS
CSCD
北大核心
2013年第1期34-41,共8页
Acta Hydrobiologica Sinica
基金
中央级公益性科研院所基本科研业务费专项(东海水产研究所2011M08)
国家公益性行业(农业)科研专项(200903048-07)
上海市长江口青草沙水库邻近水域生态修复专项资助
关键词
中华绒螯蟹
放流
肝胰腺
血清
代谢
Eriocheir sinensis
Stocking
Hepatopancreas
Serum
Metabolism
