摘要
采用世界动物卫生组织(OIE)通用的方法,建立动物模型,对鸡沙门菌恩诺沙星耐药性进行释放评估和暴露评估。通过释放评估证实,防突变浓度与最小抑菌浓度相比增高4～16倍,说明细菌极易发生突变产生耐药性,细菌在体外诱导容易产生耐药性。耐药基因可通过接合的方式进行转移。暴露评估中,感染耐药菌的小鼠与未感染小鼠接触,未经感染小鼠暴露于动物垫料及未处理的粪便中,未感染小鼠分离的沙门菌也出现耐药性,说明细菌耐药性可在动物之间、动物与环境之间转移。耐药菌易在小鼠结肠中定植。耐药菌存在扩散的风险。
Used OIE general method,animal model were established for enrofloxacin-resistant Salmonella enteritidis release and exposure assessment. Through the release assessment confirmed that MPC（mutant prevenlion concentration） increased about 4 16 times compared with MIC（minimum inhibitory concentrations） and bacteria were vulnerable to mutation produce resistance. The bacteria were easy to produce resistance in vitro induced： Resistance genes can be transfered through conjugation way. In exposure assessment, mice infected enrofloxacin-resistant bacteria contacted with not infected mice,without the infected mice exposed to animal pad and the untreat ed waste,enrofloxacin-resistant Salmonella enteritidis were separated in not infected mice. Drug resistance of bacteria can be transfer among animals and animal environment. Drug-resistant bacteria planted easily in the mice colon. The results confirmed that a risk of spread of drug-resistant bacteria may exist.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第2期254-258,共5页
Chinese Journal of Veterinary Science
基金
公益性行业(农业)科研专项经费资助项目(201203040)
关键词
沙门菌
恩诺沙星
耐药性
风险评估
Salmonella enteritidis
enrofloxacin
resistance
risk assessment
