摘要
检测了在不同给药剂量下,健康鸡服用恩诺沙星后,在不同时间粪尿中恩诺沙星及其主要代谢产物的含量变化,并对在不同光照条件下鸡粪中恩诺沙星的降解进行了研究。结果表明,健康鸡灌服恩诺沙星后,主要以恩诺沙星原形、其次以环丙沙星的形式随粪(尿)排出体外。2.5 mg/kg体重剂量组和5 mg/kg体重剂量组在给药后6h恩诺沙星的排泄量达到最高峰;环丙沙星达到峰浓度的时间为4 h。7.5 mg/kg体重剂量组恩诺沙星排泄量达到最高峰的时间为9 h;环丙沙星达到峰浓度的时间为6 h。至给药后第10天时鸡粪中检不出恩诺沙星和环丙沙星。鸡粪中恩诺沙星的降解速率受光照条件的影响,在避光条件下,鸡粪中恩诺沙星几乎很少降解;在自然光照下,鸡粪中恩诺沙星的降解较快,其变化趋势符合一级动力学方程Ct=C0e-kt,并由此求得鸡粪中恩诺沙星在自然光照下的降解半衰期为(2.23±0.25)d。
In the first part of the study, the concentration of enrofloxacin and its main metabolite in chicken feces were examined at the dosage of 2.5, 5 and 7.5 mg/kg body weight respectively by high-performance liquid chromatography （HPLC） with fluorescence detection. In the second part of the study, the degradability of enrofloxacin in chicken feces was tested under different form of illumination. The results were as follows：After administration in healthy chicken, enrofloxacin was excreted in feces as the parent compound （main form） and its metabolite as ciprofloxacin. When the oral dosage was 2.5 mg/kg and 5.0 mg/kg body weight, the concentration of enrofloxacin and ciprofloxacin in chicken feces reached the peak at the 6 th hour and at the 4 th hour after administration respectively. When the oral dosage was 7.5 mg/kg body weight, the concentration of enrofloxacin and ciprofloxacin reached the peak at the 9 th hour and at the 6 th hour after administration respectively. At 10 th day after administration, enrofloxacin and ciprofloxacin couldn＇t be detected in chicken feces. The degradation of enrofloxacin in chicken feces was affected by illumination. Enrofloxacin in chicken feces was very stable in photophobic condition. But under daylight illumination, the degradation rate of enrofloxacin was very fast in chicken feces. The degradation process of enrofloxacin in chicken feces could be evaluated on the basis of the first order kinetic equation as Ct=C0e^-kt. Calculated by this equation, the half-life of enrofloxacin in chicken feces was（2.23±0.25）d under daylight illumination.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2005年第10期1069-1074,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然科学基金重点项目(31031040)
关键词
恩诺沙星
鸡
排泄
降解
enrofloxacin
chicken
excretion
degradation
