摘要
目的探讨人工合成E-选择素对大鼠局部脑缺血再灌注损伤脑组织中血管细胞黏附分子-1(VCAM-1)及其mRNA表达的影响。方法采用改良的Zea Longa法建立脑缺血再灌注损伤模型,60只雄性SD大鼠随机分为模型组、假手术组和治疗组。采用免疫组化法观察缺血区脑组织中微血管VCAM-1阳性细胞数,RT-PCR法观察其mRNA表达。结果①模型组VCAM-1阳性细胞在再灌注2 h后开始出现,并持续增多,24 h后达高峰,72 h开始下降,各时间点比假手术组明显增多(P<0.01);VCAM-1 mRNA在再灌注2 h后开始表达,12 h后达高峰,24 h开始降低,各时间点比假手术组明显增高(P<0.01)。②假手术组VCAM-1阳性细胞数少见,VCAM-1 mRNA表达水平低。③治疗组(24 h)VCAM-1阳性细胞数比假手术组增多(P<0.05),但较模型组少(P<0.05);VCAM-1 mRNA表达比假手术组高(P<0.05),但较模型组低(P<0.05)。结论大鼠局灶性脑缺血再灌注损伤后,VCAM-1及VCAM-1 mRNA表达增多;人工合成E-选择素可降低VCAM-1及VCAM-1 mRNA的表达从而抑制白细胞与血管内皮细胞黏附,减轻炎症反应和脑缺血再灌注损伤,发挥脑保护作用。
Aim To investigate the effect of artificial synthetic E-selectin on the expression of vascular cell adhesion molecule-1 （VCAM-1） and VCAM-1 mRNA after focal cerebral ischemia-reperfusion injury in rats. Methods 60 male SD rats were randomly divided into sham operation group, model group, artificial synthetic E-selectin treated group. Modified Zea Longa＇ s method was used to establish cerebral ischemia- reperfusion injury model. 10 mg＇kg1 artificial synthetic E-selectin was injected into the femoral vein of the rats in the treated group. Immunohistochemistry was employed to observe the microvascular positive cell count of VCAM-1 in the ischemic brain tissue. RT-PCR method was used to observe the mRNA expression of VCAM-1. Results ① Model group： The VCAM-1 positive cell was found at 2 h after reperfusion, increasedwith time went by, reached peak at 24 h, and then decreased at 72 h. The expression of VCAM-1 in model group was more than sham operation group at each time point（P〈0.01）. VCAM-1 mRNA was expressed at 2 h after reperfusion, peaked at 12 h and decreased at 24 h. The expression ofVCAM-1 mRNA in model group was also more than sham operation group at each time point（P〈0.01）. ② Sham operation group： The positive cell count of VCAM-1 was very small. And the expression ofVCAM-1 mRNA was low. ③Treated group： The positive cell count of VCAM-1 was more than sham operation group（P〈0.05）, but less than model group（P〈0.05）. The expression of VCAM-1 mRNA was higher than sham operation group（P〈0.05）, but lower than model group （P〈0.05）. Conclusion ① After the formation of focal cerebral ischemia-reperfusion injury in rats, the inflammatory cytokines in the ischemic brain tissue accumulated and upregulated the expression of vascular endothelial cell surface adhesion molecule. Then it manifested as the increased expression of VCAM-1 and VCAM-1 mRNA. ② Through decreasing the expression of VCAM-1 and VCAM-1 mRNA, the artificial synthetic E-selectin inhibited the adhesion between leukocytes and vascular endothelial cells, reduced inflammatory reaction, mitigated the cerebral ischemia-reperfusion injury and protected the brain tissue.
出处
《中国临床神经科学》
2013年第1期26-31,共6页
Chinese Journal of Clinical Neurosciences
基金
江苏省医学重点学科基金项目(编号:xk2007227)
关键词
人工合成
E-选择素
脑
缺血再灌注
损伤
血管细胞黏附分子-1
artificial synthetic
E-selectin
cerebral
ischemia-reperfusion
injury
vascular celladhesion molecule-1
