摘要
目的:探讨雌激素对去势大鼠局灶脑缺血的治疗作用及其可能机制。方法:采用线栓法制备去势大鼠大脑中动脉缺血再灌注模型。经肌注苯甲酸雌二醇并观察神经功能缺损评分,损伤区组织病理学,脑梗死体积比,脑水肿体积,免疫组化方法检测雌激素受体(ER)的表达情况和血液中IL-1及TNF活性的变化。结果:雌激素组较对照组再灌注后2小时神经功能缺损评分无差异,而70小时评分有显著差异(P<0.01)。而且脑梗死体积比、脑水肿体积、血液中IL-1及TNF活性明显低于对照组(P<0.01)。大脑皮层ER(+)的神经元数量多,且胞浆阳性为主。结论:雌激素能减轻去势大鼠局灶脑缺血的脑损伤,这种保护机制可能与雌激素降低IL-1、TNF活性,影响胞浆ER表达有关。
Objective:To study the neuroprotection and its mechanism by estrogen after cerebral ischemic reperfusion in ovariectomized rats.Methods:Female SD rats bilaterally ovariectomized two weeks before were subjected to 2-hour middle cerebral artery(MCA)occlusion by intraluminal filament,followed by a reperfusion of 70 hours.The neurological status of each rat was evaluated after reperfusions of 2 hours and 70 hours.At the reperfusion 70 hours after administration of estrodiol benzoate,the cerebral pathology, change in cerebral infarct volume ratio and edema volume were investigated under microscope;immunohistochemistry technique was applied to study the expression of estrogen-receptor(ER)of cerebral cortex;the serum levels of IL-1 and TNF were measured.Results:The neurological deficit scores,cerebral infarct volume ratio,cerebral edema volume,serum levels of IL-1 and TNF at reperfusion 70 hours in the group treated with estrogen were significantly reduced than that of control group(P<0.01)except neurological deficit scores after reperfusion 2 hours (P>0.05).Furthermore,the number of ER-labeled positive cortical neurons after estrogen injection was significantly increased than that in control group.Conclusion:Estrogen provides significantly neuroprotective effect for ovariectomized rat with focal infarction.A possible mechanism of neuroprotection is by means of inhibiting inflammatory cytokines(IL-1、TNF) releasing.Potential interaction between estrogen and ER might be involved in neuroprotection induced by estrogen against cerebral ischemic injury.
出处
《华西医学》
CAS
2005年第2期295-296,共2页
West China Medical Journal
