摘要
目的研究CD40基因5'非翻译区-1位点C/T多态性在本地区哮喘儿童人群中的分布,探讨其与哮喘发病风险之间的关系。方法以134例哮喘患者作为研究对象,儿童门诊健康体检者113名作为对照组。应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测哮喘组和对照组CD40-1C/T位点的基因型及等位基因频率;采用免疫比浊法测定血清总IgE水平。分析该基因多态性与儿童哮喘发病风险及IgE水平之间的关系。结果哮喘组和对照组之间C与T等位基因频率分布差异无统计学意义(P>0.05);CC基因型与CT+TT基因型之间的分布差异有统计学意义(P<0.05),CC基因型的发病风险是CT+TT基因型的1.75倍[95%可信区间(CI):1.01～3.04]。哮喘组IgE水平在CC与CT、TT基因型间差异有统计学意义(P<0.05)。结论CD40基因5'非翻译区-1位点CC基因型可能与本地区儿童哮喘的发生有关。
Objective To investigate the distribution of CD40 gene 5＇ untranslated region-1 site C/T gene polymorphism,and to study its relationship with the risk of asthma in local asthma children. Methods A total of 134 asthma children were enrolled, and 113 healthy children from healthy examination subjects were as control group. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to determine the genotype and the allele frequencies of CD40-1C/T gene in asthma and control groups. The serum total level of IgE was determined by immunonephelometry. The relationship of CD40-1C/T gene polymorphism with the risk of asthma and IgE level was analyzed. Results The distributions of the C and T allele frequencies between asthma and control groups had no statistical significance （ P 〉 0.05 ）. The distribution difference of CC and CT ＋ TT genotype frequencies was statistically significant （P 〈0.05）. The risk of CC genotype was l. 75 times higher than that of CT ＋TT genotype E 95% confidence interval （（21） ： 1.01-3.04 ]. There were significant differences in the level of IgE between CC genotype and CT or TT genotype in asthma group （P 〈 0.05 ）. Conclusions CC genotype of CD40 gene 5＇ untranslated region -1 site might be associated with the development of asthma in local asthma children.
出处
《检验医学》
CAS
2013年第1期17-20,共4页
Laboratory Medicine
