摘要
目的研究Mina基因rs4857304（G/T）位点、IL-4基因位点rs2243248（T/G）和rs2070874（T/C）单核苷酸多态性与中国汉族儿童过敏性哮喘发病相关性,同时对3个基因位点之间交互作用进行分析。方法采用病例对照研究,MALDI-TOF技术进行IL-4和Mina位点基因分型。观察不同基因型和等位基因频率在哮喘和对照组中的分布差异,用MDR软件分析各位点之间交互作用。结果共纳入哮喘患儿202例年龄在6.97±2.85岁之间,对照组191例年龄在8.14±3.13岁之间。Mina的rs4857304位点,在哮喘组和对照组等位基因频率比较差异具有统计学意义（P=0.0199,OR=1.477,95%CI=1.062~2.052）,哮喘组rs4857304位点等位基因T频率高于对照组,而两组之间该位点基因型分布比较差异无统计学意义（P〈0.05）。IL-4基因位点rs2243248和rs2070874基因型和等位基因频率在两组比较差异均无统计学意义（P〉0.05）。MDR交互作用分析显示,IL-4基因位点rs2243248和Mina基因位点rs4857304构成的2个位点最佳模型;IL-4基因位点rs2243248、rs2070874和Mina位点rs4857304构成的3个位点最佳模型两组比较均有统计学差异（P〈0.05）。结论 Mina基因位点rs4857304与过敏性哮喘具有相关性,同时IL-4位点rs2243248,rs2070874与Mina rs4857304均具有明显交互作用。
Asthma is relative to multiple environmental factors and polygenetic backgrounds,so research on genetic background is significant to clarify the mechanism of asthma.We intended to study the associations of the polymorphisms of Mina rs4857304（G/T）,IL-4 rs2243248（T/G） and rs2070874（T/C）,and the interactions among IL-4 rs2243248,rs2070874,and Mina rs4857304 of children asthma in Chinese Han people.A case-control study was applied to observe SNPs distribution of IL-4 and Mina gene in the asthmatic patients and controls.202 cases of asthmatic children（aged 6.97±2.85 years） and 191 controls were included.SNP genotyping was performed by means of matrix assisted laser desorption ionisation-time of flight mass spectrometry method（MALDI-TOF）.Multifactor dimensionality reduction（MDR） software was used to analyze the interactions among IL-4 rs2243248,rs2070874,and Mina rs4857304 of children asthma.The results showed that among the three loci,the Mina rs4857304 of asthmatic patients presented with a significantly higher portion of T allele frequencies（P = 0.0199,vs control;OR = 1.477,95%CI = 1.062-2.052）,but no statistical differences among the genotypes frequencies of rs4857304 compared with controls（P = 0.0587）.About IL-4 gene,the genotypes and allele frequencies of rs2243248 and rs2070874 have no statistical differences between the two groups.There were significant differences in the interaction model between IL-4 and Mina gene（including 2 and 3 loci best model） analyzed by MDR（P〈0.05）.In conclusion,our results highlight the role of Mina as a susceptibility gene for children asthma,and the interactions among IL-4 rs2243248 and rs2070874 and Mina rs4857304 also have associations with children asthma in Chinese Han people.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2011年第5期416-419,共4页
Immunological Journal
