摘要
【目的】研究中草药对罗非鱼无乳链球菌和海豚链球菌的抑菌活性,为生产中罗非鱼链球菌病害的防治提供参考。【方法】采用琼脂扩散法,测定153种中草药乙醇提取物对罗非鱼(Tilapiasp.)无乳链球菌(Streptococcus agalactiae)和海豚链球菌(S.iniae)的体外抑菌作用,并采用二倍稀释法测定中草药乙醇提取物的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。【结果】153种中草药中,博落回(Macleaya cordata)、十大功劳(Mahonia fortunei)、三颗针(Berberis spp.)、紫草(Lithospermum erythrorhizon)、田七须(Panax notoginseng)、补骨脂(Psoralea corylifolia)、田基黄(Hypericum japonicum)和五倍子(Galla chinensis)提取物对无乳链球菌表现出了明显的抑制活性,其中博落回的抑菌效果最显著,抑菌圈直径为(25.1±0.6)mm;博落回、千斤拨(Flemingia philippinensis)、田七须、甘草(Bidens bipinnata)、田基黄、败酱草(Patrinina villosa)、紫草、苦参(Sophora flavescens.)、补骨脂、北五味子(Schisandra chinensis)、五倍子、鬼针草(Bidens bipinnata)、虎刺(Damnacanthus indicus)、三颗针、翠云草(B.bipinnata)、羌活(Notopterygium incisum Ting ex)、香薷(Elsholtzia patrini Garcke)对海豚链球菌具有明显的抑菌活性,其中博落回的抑菌作用最强,抑菌圈直径为(24.3±1.1)mm。对抑菌活性较强的中草药的MIC和MBC进行了测定,结果表明,三颗针和博落回提取物的抑菌和杀菌效果均较好。三颗针、博落回对无乳链球菌和海豚链球菌的MIC和MBC分别为0.31,0.63;0.63,1.25和1.25,2.50;0.94,1.88mg/mL。【结论】博落回、紫草、田基黄、补骨脂、三颗针、田七须和五倍子对无乳链球菌和海豚链球菌均具有较好的抑菌效果。
[Objective] The antimicrobial effects of Chinese herbal medicines against the pathogen of Streptococcus from tilapia in vitro were studied. [Method] The antimicrobial effects of 153 Chinese herbal medicines were screened by disc agar diffusion. Double-dilution method was used to detect the minimal in- hibitory concentration and the minimum bactericidal concentration of some herb medicines which had sig nificant inhibitory effects to streptococcus, pathogens from tilapia streptococcusis,Streptococcus iniae and S. agalactiae. [Resultl The results showed that 8 kinds of Chinese herbs including Macleaya cordata ,Ma- honia f ortunei , Lithosperrnurn erythrorhizon , Berberis spp. , Hypericurn japonicum , Panax notoginseng Psoralea corylifolia, and Galla chinensie could effectively inhibit the growth of S. iniae,their minimal in- hibitory concentrations and the minimal bactericidal concentrations were among 0.31--20.00 mg/mL and 0. 63--20. 00 mg/mL, respectively. Macleaya cordata had the best inhibitory effects with the diameter of inhibition zone（DIZ）of （24.3± 1.1） mm. Macleaya cordata , Glycyrrhiza uralensi , Lithosperrnum eryth- rorhizon , Hypericum japonicum , Panax notoginseng , Flemingia philippinensis , Patrinina villosa , Sopho- ra f lavescens , Psoralea coryli f olia , Schisandra chinensis , Bidens bi pinnata , Selaginella uncinata , Berberis spp,. and Galla chinensis inhibited the growth of S. agalactiae,their minimal inhibitory concentrations and the minimal bactericidal concentrations were 1.25--20.00 mg/mL and 2.50--40.00 mg/mL, respectively. Macleaya cordata had the strongest inhibitory effects with the DIZ of （24.3 ±1.1） mm. Berberis spp. and Macleaya cordata were the two best medicines against Streptococcus iniae and S. agalactiae. The MIC and MBC of Berberis spp. were 0.31,0.63 mg/mL（Streptococcus iniae）and 1.25,2.50 mg/mL（S, agalactiae） while that of Macleaya cordata were 0. 63,1. 25 mg/mL（Streptococcus iniae） and 0. 94, 1. 88 mg/mL （S. agalactiae）. [Conclusion] The screening results confirmed the possible use of Chinese herbal medicines as a source of antimicrobial alternatives to control the streptococcus diseases in tilapia production.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2013年第1期25-32,共8页
Journal of Northwest A&F University(Natural Science Edition)
基金
广西自然科学基金项目(桂科基0832021
桂科攻1123006-4)
中国科学院广西植物研究所基本业务费项目(桂植业10008
11009)
关键词
中草药
无乳链球菌
海豚链球菌
抑菌活性
Chinese herbal medicines
Streptococcus agalactiae
Streptococcus iniae
antimicrobiol activ- ities
