摘要
根据夹心法原理,初步建立了植物激素吲哚乙酸的酶联免疫测定法。在这个方法中,抗体最适包被浓度为20μg/ml。辣根过氧化物酶与抗体的结合物的最适稀释度为1:1000。4％聚乙二醇6000极大地改善了标准曲线,检测范围在每孔1.0至32ng。其它植物激素如脱落酸,吲哚丁酸与抗体的交叉反应很小。对植物材料中吲哚乙酸的含量进行了测定,并与其它作者用别的方法所得结果进行了比较。
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for indole-3-acetic acid determination. In this assay, the optimum coating concentration of antibod y was 20 μg/ml and the optimum dilution of enzyme-labeled antibody was 1 : 1000. Four percent PEG 6000 greatly improved the IAA standard curve. The detectability of the present ELISA ranged from 1 ng to 32 ng per well. The crossreaction of ABA and IB A with antibody was insignificant. Plant materials from sunflower were assayed for IAA and results compared with those obtained by other methods.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1991年第6期712-716,共5页
Acta Scientiarum Naturalium Universitatis Pekinensis
