摘要
以人乳腺癌组织提取的糖蛋白免疫后的BALB/C小鼠的脾细胞与小鼠骨髓瘤细胞系SP2/0细胞融合,经筛选及克隆化培养,建立了1株稳定分泌特异性抗体的杂交瘤细胞株GP-1D_8。 McAb GP-1D_8与乳腺癌组织产生强阳性反应;与正常乳腺及一些其它肿瘤组织为阴性反应;与甲状腺癌及正常结肠组织出现弱交叉反应。 经Sepharcyl-S200 HR分子筛分离的抗原分子,分子量为66kd,与Western blot测出的分子量相符。抗原分子含糖量为16％。其抗原决定簇为一段耐70℃温度的多肽。与前人报导的乳癌相关抗原有区别,可能是一种新的乳腺癌相关抗原。
Six hybridoma cell lines against human mammary tumor glycoproteins extract were raised in our laboratory. One of them (GP1D8) was investigated.McAb GP1D8 can react with 82% human breast cancer tissue sections when using the indirect immunoperoxidase histochemistry assay. It has weak reaction with human thyriod carcinoma and normal colon tissue section, and has negative reaction with normal mammary gland and other normal tissues or tumors.The McAb can recognize the 66kd band in SDS-PAGE of the extract. The value of McAb GPlD8's affinity content is 3.2×10-9M-1 by using competative ELISA. The extract of cytosol glycoproteins has five peaks when using Sepharcryl S-200 HR chro-matography, and the antigen activity is in the second peak. Other experiments show that antigen is a polypeptide with about 16% carbohydrate. It can remain its antigen activity after 30min of 70℃ treating. The practical use of McAb GP1D8 was under investigation.
出处
《北京大学学报（自然科学版）》
CAS
CSCD
北大核心
1991年第4期505-511,共7页
Acta Scientiarum Naturalium Universitatis Pekinensis
关键词
乳腺癌
糖蛋白
单克隆抗体
Monoclonal antibody
Glycoprotein
Breast cancer
