摘要
报道了一种用金标复合物放大的计时电位法测定补体C_3的电化学免疫检测方法.对影响传感器响应的因素如蛋白A固定浓度、抗体包被过程的pH及浓度、金标物混合比等进行了考察.在优化的实验条件下,传感器的信号响应和补体C_3的浓度在0.12～117.3ng/mL范围内具有良好的线性关系,检出限达0.02ng/mL.
A novel electrochemical immunoassay method based on Au nanoparticle labeled immunocomplex enlargement used for detection of complement C3 was proposed in this paper. When the aggregates formed from nano-Au labeled goat-anti-human C3 and nano-Au labeled rabbit-anti-goat IgG were immobilized on the electrode surface by sandwich method （antibody/antigen/aggregate）, the electrochemical signal of the electrode was enlarged greatly. Fabrication of the immunoelectrode and the effects of experimental conditions on the performances of immunoassay, such as concentration of protein A, pH of incubating solution and the ratio of nano-Au labeled goat-anti-human C3 to nano-Au labeled rabbit-anti-goat IgG, were dis- cussed respectively. The formed immunosensor could quantitatively determine complement C3 in the range of 0.12-117.3 ng/mL, and the detection limit was 0.02 ng/mL.
出处
《化学学报》
SCIE
CAS
CSCD
北大核心
2005年第22期2093-2097,共5页
Acta Chimica Sinica
基金
国家自然科学基金(Nos.20435010
20375012
20205004
20205005)资助项目.
