摘要
目的:建立测定复方芩苍鼻喷雾剂中黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Symmetry C18(150mm×4.6mm,5μm),流动相为甲醇-水-磷酸(47:53:0.2,V/V/V),流速为1.0ml/min,检测波长为280nm,柱温为20℃。结果:黄芩苷的质量浓度在6.2～310.0μg/ml范围内与峰面积积分值呈良好线性关系(r=0.9993);平均加样回收率为98.72%,RSD=0.89%(n=9)。结论:本方法简便、快速、准确、重复性好,可用于复方芩苍鼻喷雾剂中黄芩苷的含量测定。
OBJECTIVE： To establish a method for the content determination of baicalin in Compound qincang nasal sprays. METHODS： HPLC method was adopted. The determination was performed on Waters Symmetry C18 （150 mm×4.6 mm, 5 μm） col- umn with mobile phase consisted of methanol-water-phosphoric acid （47 ： 53 ： 0.2, V/V/V） at the flow rate of 1.0 ml/min. The detec- tion wavelength was set at 280 nm and column temperature was 20 ℃. RESULTS： The linear range of baicalin was 6.2-310.0 μg/ml （r=0.999 3） with an average recovery of 98.72% （RSD=0.89%, n=9）. CONCLUSION： The method is simple, rapid, accurate and reproducible, and it is suitable for the content determination of baicalin in Compound qincang biyan sprays.
出处
《中国药房》
CAS
CSCD
2013年第7期644-646,共3页
China Pharmacy
