摘要
目的研究肝病二号合剂能否诱导肝纤维化大鼠肝星状细胞凋亡,且能使bcl-2、bcl-2a、bax蛋白表达增加。方法雄性SD大鼠50只,体质量200g±25g,50只大鼠均用40%(V/V)四氯化碳溶液按0.15mL/100g皮下注射,每周2次,连续用药8周后,随机处死其中5只证实肝纤维化模型形成,将剩余45只随机分成空白组(A组)20只,模型组5只(C组)、治疗组(B组)20只,B组20只予肝病二号合剂4mL/(100g d)灌服8周,A组20只,用等量的生理盐水灌服8周治疗结束,C组为模型组。采用末端脱氧核苷酸转移酶介导的缺口末端标记(TUNEL)检测肝细胞凋亡,采用免疫组化法检测bcl-2 bcl-2a和bax蛋白。结果 B组与A、C组比较,肝星状细胞数量B组的细胞数量明显减少,肝星状细胞凋亡的数量明显增加,细胞凋亡指数增加明显。B组bcl-2 bcl-2a和bax蛋白明显增加。结论肝病二号合剂可以诱导肝纤维化大鼠肝星状细胞凋亡,且能使bcl-2、bcl-2a、bax蛋白表达增加。
Objective Ⅱ mixture can induce liver fibrosis in rat hepatic stellate cells,and make bcl-2,bcl-2a,bax protein expression.Methods 50 male SD rats weighing 200g ± 25g,50 rats were used 40%（V/V） carbon tetrachloride solution by 0.15mL/100g subcutaneously 2 times a week,continuous medication 8wk after random of which five were killed confirmed the formation of hepatic fibrosis model,the remaining 45 were randomly divided into blank group（A） 20,model group 5（C）,the treatment group（B） 20,B group 20 to the liver Ⅱ mixture 4mL/（100g d） fed 8wk,A group of 20,with the same amount of saline fed 8wk.the end of treatment,C group,model group.Using terminal deoxynucleotidyl transferase-mediated nick end labeling detection of liver cell apoptosis,using immunohistochemical staining bcl-2 bcl-2a and bax.Results In group B and A,C group,B group significantly reduced the number of hepatic stellate cells and apoptosis of hepatic stellate cells increased the apoptosis index increased significantly,P 0.05,B group of bcl-2 bcl-2a and bax protein increased significantly.Conclusion Ⅱ mixture can induce liver fibrosis in rat hepatic stellate cells,and make bcl-2,bcl-2a,bax protein expression.
出处
《中国医药指南》
2013年第1期400-402,共3页
Guide of China Medicine
基金
徐州市科学技术项目
项目编号X2004551
