摘要
利用激光拉曼光谱技术研究了新型光敏剂血卟啉单甲醚对 DNA(脱氧核糖核酸 )分子的光动力学损伤。结果表明 :DNA分子由典型 B型构型变成了一种修饰过的 B′型。连接碱基对的氢键被部分打断 ,碱基堆叠也有所瓦解。碱基本身也遭到了破坏 ,有脱氨基现象发生。腺嘌呤和胞嘧啶对血卟啉单甲醚的光动力学损伤更敏感。
Hematoporphyrin monomethyl ether (HMME) is a new photosensitzer with higher photosensitizing ability stronger photodynamic effects and selective uptake into tumor tissue. We analysed photodynamic damages of DNA with HMME as photosensitzer by Raman spectroscopy and obtained at following conclusions: The conformation of DNA has changed from normal B form to modified B′ form. A part of hydrogen bonds between two bases of pair were broken. The base stacking collapsed to some extent and bases were damaged. Guanine and cytosine were more sensitive to HMME.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2000年第4期529-532,共4页
Acta Optica Sinica
基金
广东省自然科学基金资助项目!(974 133)
关键词
血卟啉单甲醚
脱氧核糖核酸
光动力学损伤
拉曼
hematoporphyrin monomethyl ether, DNA, photodynamic damages, Raman spectrum.
