摘要
[目的]研究miR-34b/c启动子区单核苷酸多态rs4938723(T/C)对其转录调控的影响。[方法]构建含有不同基因型miR-34b/c启动子区的报告基因表达载体pGL3-basic-miR34b/c-T和pGL3-basic-miR34b/c-C,分别与内参质粒SV40共转染于Hela、A549和CHO三种细胞后进行荧光素酶活性分析。[结果]pGL3-basic-miR34b/c-C等位基因组荧光素酶相对活性与pGL3-promoter-miR30C-T组等位基因相比,均有显著升高(P<0.01)。[结论]荧光素酶活性强弱间接反映了miRNA启动子区遗传变异对转录活性的影响,当miR-34b/c启动子区rs4938723位点为C时,可以更为有效地增加miR-34b/c的转录产物,从而在转录水平上调控miRNA的表达。
[Purpose] To investigate the impact of a common polymorphism rs4938723（T/C） in promoter region of miR-34b/c on the regulation of miR-34b/c transcription.[Methods] The pGL3-basic-miR34b/c-T and pGL3-basic-miR34b/c-C luciferase plasmids were created containing T or C allele of miR-34b/c promoter region.Then the two plasmids contained T or C allele were transfected respectively into Hela,A549 and CHO cells to detect the transcription activity of miR-34b/c.[Results] pGL3-basic-miR34b/c-C construct group showed significantly higher levels of luciferase expression compared with pGL3-basic-miR34b/c-T construct group（P0.01）.[Conclusion] The miR34b/c transcript product could be effectively increased while the miR-34b/c carrying the rs4938723 C allele.
出处
《肿瘤学杂志》
CAS
2012年第11期835-839,共5页
Journal of Chinese Oncology
基金
国家自然科学基金重点项目(30730080)
国家自然科学青年基金项目(81102179)
