摘要
目的探讨不孕症患者在接受体外受精一胚胎移植（IVF-ET）治疗中，取卵日卵泡液内血管内皮生长因子（VEGF）的水平与妊娠结局的关系。方法对2011年7月～2012年1月接受IVF治疗周期的女性患者95例，采用酶联免疫吸附试验（ELlSA）检测取卵日穿刺留取的卵泡液中VEGF水平，及时记录相应卵子的成熟度及后续胚胎质量，并随访妊娠结局。结果成熟卵组卵泡液中VEGF浓度（2395．1±218．6pg／m1）明显高于未成熟卵（1569．4±166．2pg／m1），两者差异有统计学意义（t=14．120，P〈0．05）；优质胚胎组卵泡液中VEGF浓度（3236．7±245．2pg／m1）高于非优质胚胎组（2989．3±258．1pg／mD，两者差异有统计学意义（t=3．400，P〈O．05）；妊娠组卵泡液中VEGF浓度（4693．1±326．4pg／m1）高于非妊娠组（2666．6±233．9pg／m1），两者差异有统计学意义（t=26．118，P〈0．05）。结论卵泡液中VEGF的含量可以预测IVF—ET的结局。
Objective To study the relationship between the level of vascular endothelial growth factor （VEGF） and the out- comes of pregnancy in infertility patients undergoing IVF-embryo transfer （IVF-ET） treatment. Methods 95 cases of fe male patients undergoing IVF treatment cycle were selected from July 201t to January 2012. The enzyme-linked immunosor- bent assay （ELISA） were used to detect VEGF levels of COH Day puncture specimens in the follicular fluid, and corre- sponding eggmaturity and subsequent embryo quality were timely recorded and the outcome of pregnancy were observed fol- low-up. Results VEGF concentration （2 395.1±218.6 pg/ml） of the follicular fluid in mature eggs was significantly higher than that of the immature oocytes （1 569.4± 166.2 pg/ml）, the difference was statistically significant （t = 14. 120, P〈 0. 05）;the VEGF levels （3 236.7±245.2 pg/ml） of follicular fluid in the quality embryonic group was higher than that of the non-quality embryo group （2 989.3±258.1 pg/ml）, the difference was statistically significant （t= 3. 400,P〈0.05）. The VEGF levels （4 693.1±326.4 pg/ml） of follicle fluid in the pregnancy was higher than that of the non-pregnant group （2 666.6±233.9 pg/ml） ,the difference was statistically significant （t=26. 118,P〈0.05）. Conclusion The outcome of IVF-ET might be predicted by detecting the levels of VEGF in follicular fluid.
出处
《现代检验医学杂志》
CAS
2012年第6期139-141,共3页
Journal of Modern Laboratory Medicine
