摘要
以蛹虫草子实体为原料,通过碱法提取蛹虫草的蛋白质,以蛋白质的提取率作为提取工艺的衡量指标。在单因素实验的基础上,通过Box-Behnken模型对提取工艺进行响应曲面优化,在提取温度为60℃的条件下,确定最佳提取条件为:提取时间128 min,料液比为1∶16.2(g∶mL),NaOH溶液的浓度0.14 mol/L。在此条件下验证蛋白质的得率为88.92 mg/g与预测值88.19 mg/g接近。
Alkaline extraction method was used to extract protein from Cordyceps militaris. The protein yield was designed as the target index. Based on single-factor experiment, and the principle of Box-Behnken central composite design, response surface analysis （RSA） was applied to optimize the extraction conditions of protein from Cordyceps militaris. At the extracting temperature of 60℃, the optimum extraction conditions were： extrcating time 128 min, solid-liquid ratio 1： 16.2, NaOH solution concentration 1.4 mol/L. Under the above conditions, the highest protein yield of estimated and actual values were 88.19mg/g and 88.92% , respectively.
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2012年第12期185-188,共4页
Food and Fermentation Industries
关键词
蛹虫草
蛋白
提取
响应曲面法
Cordyceps militaris, protein, extraction, response surface anylysis
