摘要
目的:探讨喉癌组织中高迁移率族蛋白B1(HMGB1)和基质金属蛋白酶(MMP)-2、MMP-9的表达及其与临床病理特征和预后的关系。方法:应用免疫组织化学EnVision二步法检测61例喉癌组织中HMGB1和MMP-2、MMP-9蛋白表达;实时荧光定量PCR法检测30例喉癌组织中HMGB1和MMP-2、MMP-9mRNA表达。结果:HMGB1和MMP-2、MMP-9蛋白在喉癌组织中的阳性表达率明显高于癌旁正常组织(χ2值分别为44.934和49.923、36.054,均P<0.01);HMGB1和MMP-2、MMP-9mRNA在喉癌组织中的相对表达水平明显高于癌旁正常组织(t值分别为5.940和7.005、7.664,均P<0.01);HMGB1和MMP-2、MMP-9蛋白表达均与喉癌T分期、临床分期和淋巴结转移有关(P<0.05或<0.01);HMGB1和MMP-9蛋白在喉癌组织中的表达呈正相关(r=0.381,P<0.01),HMGB1和MMP-2蛋白在喉癌组织中的表达无相关性(r=0.094,P>0.05);Kap-lan-Meier生存分析结果显示,HMGB1和MMP-9表达阳性患者的生存率低于表达阴性患者(χ2值分别为4.974、6.418,均P<0.05);COX回归分析显示,HMGB1是危险因子,其表达越高,患者预后越差(P<0.05)。结论:HMGB1和MMP-2、MMP-9与喉癌的浸润、转移有关;HMGB1和MMP-9的表达有协同促进作用;HMGB1可能是影响预后的独立危险因素。
Objective：To investigate the expressions of high mobility group box B1 protein（HMGB1）,matrix metalloproteinases-2（MMP-2） and matrix metalloproteinases-9（MMP-9） in human laryngeal carcinoma and study their relationships with clinicopathological characteristics and prognosis.Method：The expressions of HMGB1,MMP-2 and MMP-9 proteins were examined with the EnVision immunohistochemical method in 61 cases of laryngeal carcinoma.The expressions of HMGB1,MMP-2 and MMP-9 mRNA were detected by real-time quantitative RT-PCR method in 30 cases of laryngeal carcinoma.Result：The positive expression rates of HMGB1,MMP-2 and MMP-9 proteins were significantly higher in laryngeal carcinoma than those in adjacent tissue（χ2=44.934,49.923 and 36.054,P0.01）.The relative expression levels of HMGB1,MMP-2 and MMP-9 mRNA in laryngeal carcinoma were significantly higher than those in adjacent tissue（t=5.940,7.005 and 7.664,P0.01）.The high level expression of HMGB1,MMP-2,MMP-9 proteins was closely associated with T stage,clinical stage and the status of lymph node metastasis（P0.05 or P0.01）.There was a positive correlation between the expression of HMGB1 and MMP-9 protein（r=0.381,P0.01）.Univatiate analysis indicated that the overall survival rate was lower in patients with a positive expression of HMGB1 and MMP-9 than those with negative expression（χ2=4.974,6.418,P0.05）.Multivariate analysis showed that HMGB1 was a risk predictor.A higher expression of HMGB1 was associated with a shorter survival time.Conclusion：HMGB1,MMP-2 and MMP-9 play a role in invasion and metastasis of laryngeal carcinoma;Also there is a synergistic effect between HMGB1 and MMP-9;Moreover HMGB1 may be a independent prognostic factor.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2013年第4期181-187,共7页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
南京市科技局社会发展项目基金资助(No:200702075)
南京市卫生局基金资助(No:YKK07071)
关键词
喉肿瘤
高迁移率族蛋白类
基质金属蛋白酶类
预后
laryngeal neoplasms
high mobility group box 1 protein
matrix metalloproteinases
prognosis
